Involvement of the cerebral cortex in progressive supranuclear palsy : immunohistochemistry and electron microscopical examination of the modified Gallyas-Braak method.

得到了以下结果：1。Gallyas-Braak制剂和使用抗tau、抗泛素和抗神经丝抗体的免疫组织化学表明，所有11例进行性核上性麻痹（PSP）患者的大脑皮层中都存在神经原纤维缠结（nft）和神经丝线。我们还在大脑皮层的胶质细胞中发现了galyas阳性和tau阳性的细胞质包涵体。常规电镜检查显示，大脑皮层中有大量由扭曲小管（TT）组成的nft，而直管型的psp - nft仅偶见。对塑料包埋的galyas切片的电镜观察表明，TT表面有致密的银粒子装饰。星形细胞胶质纤维和髓鞘对银颗粒均无亲和性。对皮质皮质退化伴神经元失色症（CND）患者进行脑内亲银性神经元包涵体（ANIs）检查；CND中ANIs的免疫组织化学和超微结构特征与psp - nft、alzheimer - nft和Pick body不同。我们的结论如下：1。TT是psp患者大脑皮层中galyas阳性神经丝的主要原纤维成分。gallas和tau阳性胶质包涵体以及psp - nft表明psp病理特异性的变性过程可能涉及大脑皮层。我们认为这些大脑皮层的结构改变可能在psp痴呆的临床表现中起一定的作用。根据细胞病理改变，PSP被认为是与CND不同的实体。

The following results were obtained :1.Gallyas-Braak preparations and immunohistochemistry using anti-tau, anti-ubiquitin, and anti-neurofilaments antibodies demonstrated neurofibrillary tangles (NFTs) and neuropil threads in the cerebral cortex of all 11 brains with progressive supranuclear palsy(PSP). We also found Gallyas-positive and tau-posirive cytoplasmic inclusions in glial cells in the cerebral cortex.2.Conventional electron microscopical examination revealed numerous NFTs composed of twisted tubules (TT) in the cerebral cortex, however, PSP-NFTs of the straight tubular profile were only occasionally found.3.Electron microscopical observation of the plastic-embeddsed Gallyas-sections demonstrated that TT were densely decorated with silver particle. Neither astrocytic glial fibers or myelin sheath showed affinity to silver particle.4.A brain with corticonigral degeneration with neuronal achromasia(CND) was examined for argyrophilic neuronal inclusions (ANIs) ; the immunohistochemical and ultrastructural profile of ANIs in CND were different from those of PSP-NFTs, Alzheimer-NFTs, and Pick bodies.Our conclusions are as follows :1.TT are the major fibrillary component of the Gallyas-positive neuropil threads in the cerebral cortex of PSP.2.Gallyas-and tau-positive glial inclusions, as well as PSP-NFTs indicate that the degenerating process specific to PSP-pathology may involve the cerebral cortex. We consider that those structural alteration in the cerebral cortex may play a certain role in clinical manifestation of dementia in PSP.3.PSP is considered to be a different entity from CND on the basis of the cytopathological alteration.