Tumor Suppressor Gene in Urogenital Carcinoma

关于许多人类癌症发生的分子生物学机制的知识正在增加。在本研究中，得到了以下结果：1)人泌尿生殖道癌CD44基因变异的基因组分析。采用RT-PCR方法扩增手术标本中提取的总RNA。扩增后的cDNA经Southern blotting分析。CD44变异在8例移行细胞癌中的6例和5例肾细胞癌中均被识别。2) CD44变异在人泌尿生殖器官癌组织中的免疫组化研究用CD44变异（V5、V6、V7、V10）单克隆抗体对正常组织和癌组织进行免疫染色。在移行细胞癌中，CD44变异，尤其是V6，在基底膜附近表达较多。3)尿液细胞CD44变异采用上述方法对尿液细胞进行southern blot分析和免疫组化研究。CD44变异也通过这两种方式在细胞中被识别出来。

The knowledge as to the molecular biological mechanism of carcinogenesis is now increasing in many human cancers. In the present research, the following results were obtained.1) Genomic analysis of CD44 variants in human urogenital carcinomaTotal RNA extracted from surgical specimen was amplified by RT-PCR method. The amplified cDNA was analized for the variants by Southern blotting. CD44 variants were recognized in 6 out of 8 transitional cell carcinoma and all of 5 renal cell carcinoma.2) Immunohistochemocal study of CD44 variants in human urogenital carcinomaNormal tissues and carcinoma tissues were imuunostained with monoclonal antibody of CD44 variants (V5, V6, V7, V10). In transitional cell carcinoma, CD44 variants, especially V6, were much expressed in the area near by basement membrane.3) CD44 variants in the cells collected from urineSouthern blot analysis and immunohistochemical study were carried out with the same methods described above in the cells collected from urine. CD44 variants were also recognized in the cells by the both way.