Histological and physicological studies on neuronal control of ovary and aviduct

为了阐明卵巢和输卵管的神经元控制，我们用抗GABA抗体或其合成酶酪氨酸羟化酶（TH）或多巴胺羟化酶（DBH）抗体免疫组化检测了神经调节剂γ -氨基丁酸（GABA）和儿茶酚胺的神经递质定位。GABA特异性染色仅限于大鼠输卵管上皮粘膜，其染色强度依次递减；肝。峡部、毡层及子宫-输卵管交界区。免疫电镜分析显示，GABA仅存在于非纤毛分泌细胞的细胞质中，而不存在于纤毛细胞中。这些结果提示，非神经元GABA系统可能调节输卵管内的输卵管液，促进大鼠输卵管受精或早期胚胎发育。葡萄糖是8细胞期和胎儿发育后胚胎发生的主要能量来源之一。葡萄糖从母体血液循环转移到胚胎或fe…更多的血是根本。应用Northern blot、免疫blot、免疫组化、光镜、电镜、包埋后法检测GLUT1在大鼠输卵管和人胎盘中的表达和定位。Northern blot和免疫blot分析均显示大鼠输卵管中存在GLUT1 mRNA和蛋白。壶腹和峡部上皮粘膜腔面可见GLUT1特异性染色。电镜分析显示，GLUT1仅存在于非纤毛分泌细胞的微绒毛表面，而不存在于纤毛细胞中。另一方面，在人胎盘中，GLUT1 mRNA和蛋白水平在妊娠期间均升高。免疫组化检查显示，GLUT1定位于细胞顶刷边缘和合体滋养细胞基底质膜。电镜分析显示，细胞滋养细胞上几乎没有GLUT1的表达。这些发现提示GLUT1可能在胎儿发育中起重要作用。少

To elucidate the neuronal control of ovary and aviduct, we examined the localization of neurotransmitters of neuromodulators such a gamma-aminobutyric acid (GABA) and catecholamine by immunohistochemistry with an anti GABA antibody or its synthesizing enzyme, tyrosine hydroxylase (TH) or dopamine beta hydroxylase (DBH) antibody. Specific staining for GABA was confined to the epithelial mucosa of rat oviduct and its intensity was gradually decreased in the following arder ; ampulla. isthmus, fimbria and utero-tubal junctional region. Immunoelectron microscopic analysis revealed that GABA was abserved only in the cytoplasm of non-ciliated secretorly cells but not in ciliated cells. These findings suggest that non-neuronal GABA system may regulate the oviduct fluid for fetilization or early embryonic development in rat oviduct.Glucose is one of mafor energy sources for embryogenesis after 8-cell stage and fetal development. The transfer of glucose from maternal circulation to embryo or fe … More tus is fundamental. We examined the expression and localization of GLUT1 in rat oviduct and human placenta by Northern blot and immunoblot analyzes and immunohistochemistry using both light and electron microscopy with post-embedding method. Northern blot and immunoblot analyzes both showed the presence of GLUT1 mRNA and protein in rat oviduct. Specific staining for GLUT1 was observed in the luminal surface of the epithelial mucosa of ampulla and isthmus. Electron microscopic analysis revealed that GLUT1 was observed only on the surface of the microvilli in non-ciliated secretory cells, but not in ciliated cells.On the other hand, in human placenta both GLUT1 mRNA and protein levels increased during pregnancy. Immunohistochemical examination showed that GLUT1 was localized on the apical brush border and along the basal plasma membrane of the syncytiotrophoblast. Electron microscopic analysis revealed that GLUT1 was hardly observed on the cytotrophoblasts. These findings suggest that GLUT1 may play an important role in fetal development. Less