Proliferation and differention of oogonia in Drosophila oogenesis

在许多动物中，配子发生发生在种系囊肿中。果蝇卵母细胞在包含16个卵母细胞的囊内发育，这些卵母细胞通过细胞间桥相互连接。胞质结构，即多丝体，贯穿囊肿内的细胞间桥。为了深入了解多聚丝体在控制囊肿形成和每个囊肿内细胞质不对称中的作用，我们使用单克隆抗体对果蝇多聚丝体的一个成分进行了分子分析。本研究的新发现如下：1。多聚体含有细胞骨架蛋白。在早期卵巢囊肿中发现梭体成分分布不均。这是在囊肿中最早观察到的分子不对称。在雄性生殖系细胞和雌性生殖系细胞中均发现多丝体。观察了雄性和雌性多丝体的形态差异。这些差异受种系性别决定基因的控制。在雄性和雌性种系干细胞中也含有梭体成分。干细胞的细胞极性可以通过细胞中fusome的不对称分布来识别。

In many animals gametogenesis takes place in germline cysts. Drosophila oocytes develop within cysts containing 16 oogonial cells that are interconnected by intercellular bridges. A cytoplasmic structure, polyfusome, extends through each intercellular bridge in the cyst. To gain insight into the role of polyfusome in controlling the cyst formation and the cytoplasmic asymmetry within each cyst, we have carried molecular analyzes on a component of Drosophila polyfusome using a monoclonal antibody. New findings obtained in this study are as follows.1. Polyfusome contained cytoskeletal proteins.2. The unegual distribution of the fusomal component was found in the early ovarian cysts. This is the earliest molecular asymmetry observed in the cyst.3. Polyfusome was found in the male germline cells as well as in the female germline cells.4. Morphological differences between male and female polyfusomes were observed. These differences were under the control of the germline sex determination genes.5. The fusomal component was also contained in male and female germline stem cells. The cellular polarity in stem cell could be recognized by the asymmetrical distribution of the fusome in the cell.