Spectroscopy on Structural Formation Dynamics of Condensed Matters based on Phase Transition Phenomena

(1)凝胶体积相变中的分子动力学研究用精密温控器测量了掺杂在凝胶中的发色团在聚n -异丙基丙烯酰胺凝胶体积相变附近的荧光光谱和发射各向异性光谱。从他们的数据分析，我们得到了一个事实，即发色团的旋转运动在凝胶的体积相变之前比在体积相变之后要慢。由此我们发现凝胶的临界温度与凝胶交联密度的立方根成正比。(2)用图形法研究体积相变与相分离过程的竞争速率用摄像机测量了聚n -异丙基丙烯酰胺凝胶在临界温度以上的时间分辨形态变化。从他们的数据分析，我们得到了一个事实，即随着温度的升高，凝胶的增白率增加。(3) DNA在金属离子水溶液中螺旋线圈转变过程的光谱研究在30-9偶联色谱范围内，测量了DNA中掺杂的发色团在DNA螺旋线圈转变附近的荧光光谱和发射各向异性谱。从他们的数据分析中，我们发现DNA的螺旋线圈转变过程取决于缓冲溶液和金属离子的成分。

(1) Study on Molecular Dynamics in Volume Phase Transition of GelsFluorescence spectra and emission anisotropy apectra of chromophore doped in gels have measured in the vicinity of volume phase transition of poly (N-isopropylacrylamide) gels with precise temperature controllor. From the analysis of thier data, we obtained the fact that the rotational motion of chromophore before volume phase transition of gels was slower than that after one. Hence we found out that the critical temperature of gels was proportional to the cube root of crosslink-density of gels.(2) Rate Study of Competition between Volume Phase Transition and Phase Separation Process by Graphics MethodThe time-resolved form change of poly (N-isopropylacrylamide) gels at above critical temperatures has measured by video camera. From the analysis of thier data, we obtained the fact that the whitening rate of gels was increasing with increasing temperature.(3) Spectroscopy of Helix-Coil Transition Process of DNA in Metal Ion Aqueous SolutionFluorescence spectra and emission anisotropy sepctra of chromophore doped in DNA have measured in the vicinity of helix-coil transition of DNA in the range of 30-9ﾟC.From the analysis of thier data, we found that helix-coil transition processes of DNA depended on the component of buffer solutions and metal ions.