The Rule of Anaerobic Bacteria on Oral Infectious Diseases Image Processing of the Cell Surface of Prevotella intermedia

1)利用LUZEX-FS图像处理器对革兰氏阴性厌氧菌、中间普雷沃菌ATCC 25611、ATCC 33563和牙龈卟啉单胞菌381进行阴影处理和阈值设置后的细胞包膜结构进行检测。细菌样品用戊二醛- oso_4 （GA-OSO_4）固定，用oso4 -丙酮（FS）冷冻取代。经GA-OsO_4和FS处理，菌株25611的细胞包膜宽度分别为39.3 nm和31.5 nm；菌株381的细胞包膜宽度分别为35.8、37.0和35.8 nm。经GA-OsO_4固定（FS固定）的菌株25611细胞包膜各向宽分别为细胞膜13.0 (11.9)nm、内小叶4.6 (3.8)nm、内外小叶2.7 (4.4)nm、外小叶3.6 (3.3)nm、肽聚糖层6.2 nm、外膜9.2 (11.0)nm。GA-OsO_4对细菌的外膜间隙（即细胞膜外小叶与外膜内小叶之间）的宽度大于外膜间隙。2)利用图像处理器研究宿主细胞中细菌的超微结构，了解口腔传染病的发生。电镜下取脓肿患者的脓液。经厌氧培养和电镜观察，几乎所有脓肿均检出革兰氏阳性和阴性菌。革兰氏阴性菌与吞噬体的面积比为33.0%，革兰氏阳性菌为43.8%。革兰氏阴性菌和革兰氏阳性菌的面积比分别为33.0%和26.7%。革兰氏阴性菌外膜囊泡直径约20.6 nm，面积约541 nm ^2。溶酶体的总面积约为宿主细胞横截面面积的20.5%，而吞噬体的面积为21.1%。我们发现图像处理系统可以用于测量方面，并且可以使用数字图像处理器定量表达抗性和致病性因素的程度。少

1) We measured the cell envelope structures of Gram negative anaerobic bacteria, Prevotella intermedia ATCC 25611 and ATCC 33563, and Porphyromonas gingivalis 381 after shade processing and threshold setting using the LUZEX-FS image processor. Bacterial samples were fixed by glutaraldehyde-OsO_4 (GA-OSO_4) and freeze substitution using OsO4-acetone (FS).The width of cell envelope (between inner leaflet of cell membrane and outer leaflet of outer membrane) of 25611 strain was 39.3 nm by GA-OsO_4 and 31.5 nm by FS,and those of 381 strain were 35.8,37.0 and 35.8 nm, respectively. The width of each aspect of the 25611 strain cell envelope by GA-OsO_4 fixation (FS fixation) was 13.0 (11.9) nm for the cell membrane, 4.6 (3.8) nm for the inner leaflet, 2.7 (4.4) nm between the inner and the outer leaflets, 3.6 (3.3) nm for the outer leaflet, 6.2 nm for the peptidoglycan layr, 9.2 (11.0) nm for the outer membrane. The width of periplasmic space beside cell membrane (between the outer leaflet o … More f the cell membrane and the inner leaflet of the outer membrane) of bacteria by GA-OsO_4 was larger than that beside outer membrane.2) We studied the ultrastructure of bacteria in host cells to understand the appearance of oral infectious diseases using the image processor. Electron microscopic samples were taken of pus from patients with abscesses. Gram positive and negative bacteria were detected in almost all the abscesses used after anaerobic culture and electron microscopic observation. The area-ratio of bacteria to phagosomes was 33.0 % for gram negative, and 43.8 % for Gram positive bacteria. The area-ratio of the capsule to bacteria was 33.0 % for Gram negative, and 26.7 % for Gram positive bacteria. Vesicles originating from the outer membrane of Gram negative bacteria were about 20.6 nm in diameter and had an area of 541 nm ^2. The total lysosome area was about 20.5 % of the host cell cross section area, while this area was 21.1 % foe the phagosome.We found that the image processing system could be used to measure aspects and the degree of resistant and pathogenic factors could be expressed quantitatively using the digital image processor. Less