Effects of midazolam and flumazenilon autonomic nervous activities

采用心率波动、皮血流量波动和血压计血压波动的功率谱分析，研究咪达唑仑和氟马西尼对自主神经活动的抑制作用。健康的年轻男性志愿者参加了两个不同的阶段；M组仅给予0.07 mg/kg咪达唑仑，MFmz组在注射相同剂量咪达唑仑18 min后给予氟马西尼0.3 mg。用SRR-5 （GMS公司）记录RR间期，用AFL2激光多普勒流量计（Advance公司）测量手背血流。连续的模拟数据通过A/D转换器输入计算机PC-9801BA2 （NEC公司），数据被分成60秒的子序列，用Jentow 7700（日本浩林公司）记录血压计血压，用自主神经系统软件包（日本浩林公司）分析。采用MemCalc系统（Suwa Trust Co.），采用最大熵法（MEM）进行分析。在RR区间和血压计中，功率谱密度分为4个分量（0.04 ~ 0.4 hz）；在血流中，功率谱密度分为6个分量（0.04 ~ 2.2 hz）。两组在5 min时RR区间0.04 ~ 0.4 hz的功率下降到基线值的27 ~ 50%（平均值）。在MFmz组，在20分钟内，下降的功率增加到127-150%。在血流量方面，两组在5 min时0.1 ~ 0.20 hz的功率均下降至基线值的26 ~ 77%。MFmz组在20 min时两者均升高。在血压计中也观察到同样的趋势。表明咪达唑仑主要抑制低频功率，即对交感神经活动的抑制大于副交感神经活动，而氟马西尼对这种抑制有拮抗作用。

The effect of midazolam and flumazenil on depression of autonomic nervous activities were studied using power spectral analysis of fluctuations in heart rate, cutaneous blood flow and tonometry blood pressure.Healthy young male volunteers participated in two different sessions ; Group M received 0.07 mg/kg of midazolam only, and in Group MFmz, flumazenil 0.3 mg was administered 18 min after the injection of the same dose of midazolam. RR interval was recorded with SRR-5 (GMS Co.), and blood flow at the dorsum manus was measured with a laser doppler flowmeter AFL2 (Advance Co.). The successive analogue data were inputted via an A/D converter into a computer PC-9801BA2 (NEC Co.), and data were divided into subseries of 60 sec. Tonometry blood pressure were recorded with Jentow 7700 (Nihon-Khorin Co.) and analyzed by Autonomic Nervous System Package (Nihon-Khorin Co.). Analysis was carried out by the maximum entropy method (MEM) using MemCalc system (Suwa Trust Co.). Power spectrum densities were separated into four components (0.04-0.4Hz) in RR intervals and tonometry blood pressure, and into six (0.04-2.2Hz) in blood flow.The powers of 0.04-0.4Hz in RR intervals decreased to 27-50% (mean) of the basline value at 5 min in both groups. In Group MFmz, the decreased powers increased to 127-150% at 20 min. As to blood flow, powers of 0.1-0.20Hz decreased to 26-77% of the basline value at 5 min in both groups. In Group MFmz both values increased at 20 min. The same trend was obsewrved in tonometry blood pressure.It is indicated that midazolam depresses mainly the powers of low frequency, which means it depresses sympathetic nervous activity more than parasympathetic activity, and flumazenil antagonizes the depression.