Clinical Significance of Platelet Allo-antibodies.

1)我们无法用我们的血小板检测日本人的Mo^a， Sr^a或Va^a抗原。相比之下，约5%（15/314）被发现为Tu^a阳性。2)新生儿同种免疫性血小板减少症29例：抗hpa -4b （YuK^a）抗体20例，抗hpa -4a （YuK^ b）抗体3例，抗hpa -3a （Bak^a）抗体5例，抗hpa -5b （Br^a）抗体1例。其中合并颅内出血3例。3)通过大量样本的筛选，我们发现抗hpa -2b （Sib^a）在血小板输注难耐病例中起重要作用。4)混合被动血凝法（MPHA）是15年前发展起来的一种检测抗血小板抗体的简便、灵敏的方法。现在它已经被改进了。使用5%的蔗糖，血小板固定板可以冷冻（低于40℃）保存一年以上而不丧失反应性。因此，MPHA应作为医院任何实验室检测血小板同种抗体的常规检查。

1) We were unable to detect Mo^a, Sr^a or Va^a antigens among Japanese using our platelet-panel. In contrast, about 5% (15/314) were found to be Tu^a positive.2) We have experienced 29 cases of NAIT (Neonatal Alloimmune Thrombocytopenia) : 20 cases due to anti-HPA-4b (YuK^a) antibodies, 3 cases due to anti-HPA-4a (Yuk^b), 5 cases due to anti-HPA-3a (Bak^a) and 1 case due to anti-HPA-5b (Br^a). Among them 3 cases were complicated with intracranial hemorrhage.3) By screening a large number of samples, we found that anti-HPA-2b (Sib^a) playd an important role in the case of platelet transfusion refractoriness.4) The MPHA (mixed passive hemagglutination) method was developed about 15 years ago as a simple and sensitive test for the detection of anti-platelet antibodies. It has been refined now. Using 5% sucrose, the platelet-fixed plate can be kept frozen (below-40 C) without loss of reactivities for more than a year. Thus, MPHA should be employed as the routine test in any lab in the hospital for the detection of platelet allo-antibodies.