Preparation of Amphiphilic Chromatography Packings Composed of Polypeptides

首次用聚（< γ > -甲基或< γ > -苄基-谷氨酸）单独制备了平均直径为5-300 <微> m的多孔球，并对其进行了凝胶渗透色谱性质的研究。将多肽悬浮液中的溶剂逐渐去除，制备多肽微球。通过调节多肽溶液和悬浮介质的粘度，以及在球化（凝固）过程中选择额外的稀释剂，可以很容易地控制颗粒的大小和孔隙度。聚（< γ > -甲基l -谷氨酸）球不仅可以承受非常高的流速，而且在水体系和有机体系中都表现出典型的凝胶渗透色谱行为。这些良好的性能是由于分子间和分子内的氢键基于特定多肽构象的形成。用我们独特的方法制备了用于高压液相色谱的新型球形和大孔颗粒。该填料由聚γ - > -甲基-l -谷氨酸组成，并通过部分交联和长链烷基化进行功能化。填料非常坚硬，表现出令人满意的色谱性能。这是由于多肽单元和长链烷基分别引入了合适的极性和疏水性。

Porous spheres with average diameters of 5-300 <micro> m have been prepared for the first time from poly( <gamma> -methyl or <gamma> -benzyl L-glutamate) alone, and their gel permeation chromatographic properties, both without further modification and after cross-linking with appropriate agents, have been examined. The polypeptide spheres were prepared by gradually removing the solvent from the suspension particles containing polypeptide. The particle size and porosity were controlled with ease by adjusting the viscosity of the peptide solution and suspension medium, and by selecting additional diluents in the sphering (solidification) process, respectively, The spheres of poly( <gamma> -methyl L-glutamate) can not only withstand a remarkably high flow-rate, but also show typical gel permeation chromatographic behaviours in both aqueous and organic systems. These good properties are attributable to inter- and intramolecular hydrogen bonding based on the formation of specific polypeptide conformations.Novel spherical and macroporous particles for the high pressure liquid chromatography were prepared by our unique method. The packing was composed of poly- <gamma> -methyl-L-glutamate, and were functionalyzed by the partial cross-linking and long-chain alkylation. The packings are very rigid and show satisfactorily chromatographic properties. They are attributable to the suitable polarity and hydrophobicity introduced by polypeptide-units and long-chain alkyl groups, respectively.