Nutritional physiological studies on the pancreas digestive enzyme secretion system in chicks.

建立了一种新的鸡胰液收集方法。翼静脉注射胆囊收缩素对胰腺酶分泌有直接影响，这种反应呈剂量依赖性，似乎与0.06和0.15 IU的剂量平行，而0.30 IU CCK似乎具有选择性刺激，有利于胰凝乳蛋白酶原的最大增加，其次是淀粉酶和胰蛋白酶原。一个稳定的，相对较小的基础输出淀粉酶，胰蛋白酶原和胰凝乳蛋白酶原发生时，最终产品的消化（葡萄糖和各种氨基酸，除苯丙氨酸）是单独管理。苯丙氨酸注射液能显著刺激淀粉酶和胰蛋白酶原的分泌。当单个氨基酸与胆囊收缩素注射时，胰腺酶分泌被协同刺激，并且每种酶的反应取决于注射的氨基酸的种类而不同。这些结果表明，CCK单独或与消化终产物组合的翼静脉注射具有特定的酶分泌的特异性诱导。饲喂含10或100 ppm大豆胰蛋白酶抑制剂（SBTI）的饲料的雏鸡显示出相似的体重增加，但胰腺比饲喂不含SBTI的饲料的雏鸡重。较重的胰腺腺泡细胞肥大，这是由于粗面内质网增大和数量增加。饲喂SBTI的雏鸡酶原颗粒数量较少，但有增大的趋势。提示SBTI引起腺泡细胞的变化是通过酶原颗粒的过度分泌引起的。饲喂SBTI的雏鸡胰腺消化酶分泌反应模式与未饲喂SBTI的雏鸡相似。研究了饲以SBTI雏鸡胰腺消化酶同工酶的变化。

A new collection method for pancreatic juice in chickens was developed. The wing vein injection of cholecystokinin had an immediate effect on pancreatic enzyme secretion and this response was in a dose dependent fashion, which seemed to be parallel to a dose of 0.06 and 0.15 IU, while 0.30 IU CCK seemed to have a selective stimulation favoring a greatest increase in chymotrypsinogen followed by amylase and trypsinogen. A steady, relatively small basal output of amylase, trypsinogen and chymotrypsinogen occured when the end products of digestion (glucose and various amino acids except phenylalanine) was administered individually. Phenylalanine injection stimulated significantly the secretion of amylase and trypsinogen. When the individual amino acid was injected with cholecystokinin, the pancreatic enzyme secretion was stimulated synergetically and the response of each enzyme was different depending on the kind of amino acid injected. These results suggested the wing vein injection of CCK alone or in combination with end products of digestion had the specific induction of a particular enzyme secretion. Chicks fed a diet with 10 or 100 ppm soybean trypsin inhibitor (SBTI) showed a similar body weight gain, but had heavier pancreas than chicks fed a diet without SBTI. The heavier pancreas showed hypertrophy in acinar cells which was due to enlargement and increased number of rough endoplasmic reticulum. The zymogen granule of chicks fed SBTI was small in number but showed enlargement tendency. It was suggested that the change of acinar cell by SBTI was introduced by oversecretion of zymogen granules. The pancreatic digestion enzyme secretion response pattern of chicks fed SBTI was similar to that of chicks fed no SBTI. Isozymes of pancreas digestive enzymes of chicks fed SBTI was studied.