Diversity of functions and surface structures of Trypanosoma cruzi trypomastigotes

1. 克氏锥虫的毒力不是由它们的穿透能力引起的，而是取决于它们以下步骤的速度。高毒力trypmasastigotes渗透小鼠巨噬细胞或新生小鼠新培养成纤维细胞的速度与低毒力的速度几乎相同，但它们会迅速改变形态并开始增殖，而低毒力的则需要超过48小时的延迟时间才能增殖。在对已培养成纤维细胞（l细胞）的感染实验中未发现这种现象。锥乳虫细胞表面有30多种蛋白抗原，它们的质量和数量都不突出。因此，免疫小鼠血清不能凝集活的锥乳线虫，尽管它们对它们显示出相当高的间接免疫荧光滴度。脂类成分主要出现在高毒力的锥乳线虫上。用SDS-PAGE对经胰蛋白酶处理的不同毒力活锥乳线虫分离组分进行了比较分析。当凝胶在12.5%的TCA中固定时，显示出Mr范围为37,000至50,000的白色沉淀，但当样品用丙酮预处理时，它们消失了。

1. Virulence of Trypanosoma cruzi trypomastigotes is not caused by their penetrating abiliti but is dependent on their speed of the following steps. High-virulent trypomastigotes penetrated mouse macrophages or newly cultured fibroblasts from newborn mice at the almost same speed as low-virulent ones, but they promptly change form and start proliferation, while low-virulent ones require more than 48 hours lag time before proliferation. Tthis phenomenon is not found in the infectious experiment to the established fibroblasts (L-cells).2. There are more than 30 protein antigens on the cell surface of trypomastigote, none of which are prominent in quality and quantity. Thys, immune mouse sera can not agglutinate living trypomastigotes, even though they show considerable titer of indirect immunofluorescence to them.3. Lipid-like compoments predominantly appear on high-virulent trypomastigotes. Separated compoments from living trypomastigotes of different virulence by trypsin treatment are comparatively analysed on SDS-PAGE. White precipitates with a Mr range 37,000 to 50,000 were shown when the gels were fixed in 12.5% TCA but they disappeared when the samples were pre-treated with acetone.