Study on the pathogenesis and biological vehavior of malignant giant cell tumor of bone

按顺序研究骨骼的巨型细胞肿瘤（GCT）的病理学和细胞功能研究，用石蜡embeded、Frozen和/或调用的GCT的文化材料、恶性巨型细胞肿瘤（M·GCT）和恶性纤维细胞组织（MFH）......。这个肿瘤与病理学中GCT的密切关系经常受到讨论。《实验研究I》（1985年），我们成功地连续地从裸鼠中提取出人类GCT，并稳定了移植线。利用这种材料、细胞病理学（电子显微镜）和细胞功能学（噬细胞学、免疫生物化学）。<alpha>-1反加密素，<alpha>-1反加密素，以及类似的）观察是制造的。这项研究的结果表明，这种肿瘤中的巨型细胞是由胶原细胞的凝聚力和将基质细胞转化为历史学线上的生长，实验研究II （1986年），在t中的形态化和存在的历史学字符。 ... More 他移植了细胞，并研究了胶原酶活动。Markedly的高活跃性表现在那些细胞中，与其他肿瘤相比，他们的文化呼叫。由于最近发现的GCT材料（16个案例）超过有意义的细胞病理学和细胞功能观测已经被带走。噬细胞病和正免疫性化学支架在巨型细胞和类固醇或立方体星形细胞中经常发现，它们属于起源的历史性细胞口香糖。实验研究III （1987年） MFH被强烈地支持由一些作者产生的历史性起源。这种肿瘤细胞的特征（MFH的13个案例）和两种文化纤维块作为控制（纤维化和正常人类纤维块）已经研究了GCT组中相同的方法。本研究的结果显示出对GCT或M. GCT的强烈影响。为了证明这三年来的均匀循环的稳定性证据，GCT已经聚集在一起。他们中的任何一个人都足以直接证明和舒适。在我们需要进行持续的研究之前，我们需要一个综合性的提醒，使GCT起源于历史细胞组。Less（低）

In order to study on the pathogenesis of giant cell tumor of bone(GCT), cytomorphological and cytofunctional studies with paraffin embbeded, frozen and/or call cultured materials of GCT, malignant giant cell tumor of bone(M・GCT) and malignant fibrous histiocytoma(MFH.... the close relationship of this tumor to GCT in pathogenesis is often discussed.) were undertaken.Experimental study I (in 1985) We have successfully continued transplantation of tumor cells derived from human GCT in nude mice and established a transplantation line. With this material, cytomorphological (electron microscopic) and cytofunctional(phagocytotic, immunohistochemical.... <alpha>-1 antitrypsin,<alpha>-1 antichymotrypsin and so on) observations were made. The results of this study implied that a giant cell of this tumor was made by coalescence of stromal cells and the stromal cell took its rise in histiocytic line.Experimental study II (in 1986) In order to emphasize th existence of a histiocytic character in t … More he transplanted cells, collagenase activity was studied. Markedly high activity was shown in those cells compared to cultured call of other tumors. For newly obtained materials of GCT (16 cases) above mentioned cytomorphological and cytofunctional observation were carried out. Phagocytosis and positive immunohistochemical stain were often found in giant cells, and asteroid or cuboid stromal cells, which alluded that they belonged to histioctic cell goup in origun.Experimental study III (in 1987) MFH is strongly supposed to be histiocytic origin by some authors. Characters of this tumor cells(13 cases of MFH) and two kinds of cultured fibroblasts as a control(fibromatosis and normal human fibroblast) were studied by the same method done in GCT group. The results of this study showed strong resemblance to that with GCT or M・GCT. For these three years eloquent circumstantial evidences telling the histogenesis of GCT have been gathered. However none of them is direct proof and convincing enough. Continuous studies is needed before we make a conclusive remark that GCT originate from histiocytic cell group. Less