SEROEPIDEMIOLOGICAL AND VIROLOGICAL STUDIES OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS(LCMV) IN LABORATORY ANIMALS.

采用间接荧光抗体法检测日本实验动物菌落中淋巴细胞性脉络丛脑膜炎病毒（lcmv）抗体。结果表明，该抗体在SPF小鼠（3/152,2.2%）和普通小鼠（30/539,5.6%）中均存在，低效价范围为1:10 ~ 1:160。叙利亚仓鼠阳性率为2.2%(2/89)，黑线姬鼠阳性率为2.9%(2/68)，日本收获鼠阳性率为21.4%（3/14）。此外，在日本西部地区捕获的几种啮齿动物中，抗体阳性率为8.6%（19/222）。但在沙鼠、鼠鼠、豚鼠和大鼠的血清中尚未发现该抗体。提示日本及西方国家的实验动物和野生啮齿动物均存在LCMV感染，并指出在实验动物和野生啮齿动物中开展LCMV微生物监测的重要性。病毒分离试验结果为阴性。今后应进一步开展野生啮齿动物LCMV的再分离研究。

INDIRECT FLUORESCENT ANTIBODY METHOD WAS APPLIED FOR A DETECTION OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS(LCMV) ANTIBODY IN COLONIES OF LABORATOREY ANIMALS IN JAPAN. THE RESULTS SHOWED THAT THE ANTIBODY EXIST IN SPF MICE(3/152, 2.2%) AND CONVENTIONAL MICE(30/539, 5.6%) WITH LOW TITERS RANGING FROM 1:10 TO 1:160. THE ANTIBODY WAS ALSO DETECTED IN 2.2%(2/89) OF SYRIAN HAMSTERS, AND 2.9%(2/68) OF APODEMUS AGRARIUS, 21.4%(3/14) OF JAPANESE HARVEST MICE WHICH HAVE BEEN MAINTAINED AS LABORATORY COLONY FOR SEVERAL YEARS. FURTHERMORE, 8.6%(19/222) ANTIBODY POSITIVE ANIMALS WITH COMPARATIVE TITERS WERE DETECTED IN THE SEVERAL SPECIES OF RODENTS CAPTURED IN WESTERN PARTS OF JAPAN. HOWEVER, THE ANTIBODY WAS NOT DEMONSTRATED IN THE SERA OF MONGOLIAN GERBIL, SUNCUS MURINUS, GUINEA PIG AND RAT, THUS FAR. THESE RESULTS SUGGEST THAT LCMV INFECTION IS PRESENT IN LABORATORY ANIMALS AND WILD RODENTS IN JAPAN AS WELL AS WESTERN COUNTRIES, AND POINTED OUT THE IMPORTANCE OF MICROBIOLOGICAL MONITORING FOR LCMV IN LABORATORY AND WILD RODENTS. A TRIAL OF VIRUS ISOLATION WAS RESULTED IN NEGATIVE. REISOLATION STUDY OF LCMV FROM WILD RODENTS SHOULD BE CONDUCTED IN NEAR FUTURE.