Chemical structures of carbohydrate epitopes of host cell membrane recognized by fimbriae of pathogenic bacteria

1.肾盂肾炎大肠coliP菌毛-- E.从人肾盂肾炎尿液中分离到的大肠杆菌中，有许多P菌毛能识别CTH（P^k抗原）、球糖苷（P抗原）和P_1糖脂（P_1抗原）的Gal-4Gal序列。我们发现，一些E。具有相同菌毛抗原的大肠杆菌菌株识别Forssman糖脂，而不识别任何P抗原糖脂，其它菌株对Forssman糖脂的识别能力强，对CTH和globoside的识别能力弱。这些菌毛粘附素不同于P粘附素.产肠毒素大肠coli K99菌毛--K99菌毛E.大肠杆菌是小牛和仔猪腹泻的病原体。该菌毛粘附素可识别宿主肠粘膜的N-羟乙酰神经氨酰-乳糖神经酰胺（N-glycolylneuraminyllactosylceramide，GM_3，NeuGc）。新生犊牛和仔猪肠粘膜中均有GM_3（NeuGc）的表达，但在生后2周时已完全消失。通过重组DNA技术，将粘附因子富集到重组菌中。它适用于菌毛接种。肾伞棒状杆菌C.肾是牛肾盂肾炎的病原体。细菌以菌毛附着于尿道上皮。我们制备了针对菌毛的单克隆抗体，它可以抑制与宿主细胞的粘附。单克隆抗体识别48000菌毛蛋白。重组DNA技术分析表明，C.肾染色体DNA编码相同的蛋白质产物。

1. Uropathogenic E. coli P fimbriae -- E. coli with P fimbriae which recognize Gal -4Gal sequence of CTH(P^k antigen), globoside (P antigen) and P_1 glycolipid (P_1 antigen) were frequently isolated from human pyelonephitis urines. We identified that some E. coli strains with same P fimbrial antigen recognize Forssman glycolipid but not any Pantigenic glycolipid and other strains recognize potently Forssman glycolipid and weakly CTH and globoside. These fimbrial adhesin was different from P adhesin.2. Enterotoxigenic E. coli K99 fimbrial--K99 fimbrial E. coli is a causative agent of calf and piglet diarohea. This fimbrial adhesin was found to recognize N-glycolylneuraminyl-lactosylceramide, GM_3(NeuGc) of host intestinal mucosa. GM_3(NeuGc) was detected in intestinal mucosa of newborn calf and piglet but it completely disappeared at the 2nd week after birth. The adhesin factor was concentrated in recombinant bacteria by recombination DNA technique. It became applicable for the fimbrial vaccination.3. Corynebacterium renale fimbriae--C. renale is a causative agent of bovine pyelonephritis. The bacteria adhere to urinary track epitherium with their fimbriae. We prepared monoclonal antibodies to the fimbriae, which can inhibit the adhesion to host cells. The monoclonal antibody recognized 48000 fimbrial protein. Recobination DNA technique alsp showed that 1.4K bp of C. renale chromosomal DNA codes the same protein product.