ANALYSIS OF PURE PANCREATIC JUICE IN PANCREATIC DISEASES AND ITS CLINICAL SIGNIFICANCE

我们通过EIA检测乳铁蛋白（LF）水平，以及内镜下抽吸胰液（PJ）中的常规参数，并评估PJ中的蛋白塞（PP）。此外，测量PJ中癌症相关碳水化合物抗原，并使用抗体对胰腺标本进行免疫组织化学染色。结果总结如下：在46%的慢性胰腺炎（CP）患者中观察到PJ中异常高水平的LF，其中钙化CP（82%）的患病率明显高于非钙化CP（37%）。此外，PJ中的LF水平随着CP的严重程度而升高。由于55%的CP患者在PJ中发现了PP，而除胰腺癌（8%）外的其他疾病患者中没有发现PP，因此PP被认为是CP的相对特异性。钙化CP中PJ中PP的发生率（82%）高于非钙化CP（55%）。p阳性组与p阴性组相比，p阳性组的p、p、p、p、p、p、p、p均有显著性差异，p阳性组的p、p、p、p均有显著性差异。PJ中CA 19-9的表达水平不仅在胰腺癌（PC）中显著升高，在CP中也显著升高，其在PC中的表达率为92%，在CP中为85%。PJ中CA 19-9被认为是非特异性胰腺损伤的敏感标志物，而不是肿瘤的敏感标志物。PJ中ST-439和SLX浓度仅在PC中显著升高，阳性率分别为57%和40%。具有较高的肿瘤特异性，但发病率相对较低。CA 19-9和CA-50是1型链碳水化合物抗原，在胰腺癌中表达强烈，但特异性较低。而CSLEX-1和SLX等2型链碳水化合物抗原的肿瘤特异性最高，发生率低于其他抗原。另一方面，ST-439和DU-PAN-2表现出较高的阳性和特异性。CA 19-9强烈且广泛分布于CP的增殖性小胰管，这可能反映了CP患者PJ中CA 19-19水平升高

We determined lactoferrin (LF) levels by EIA, as well as conventional parameters in endoscopically aspirated pancreatic juice (PJ), and evaluated the protein plug (PP) in PJ. Furthermore, cancer-associated carbohydrate antigens in PJ were measured, and immunohistochemical staining of pancreatic specimens was performed using antibodies. The results are summerzied as follows.Abnormaly high levels of LF in PJ were observed in 46% of the patients with chronic pancreatitis (CP), with the prevalence being significantly higher in calcified CP 82%) than in non-calcified CP (37%). Moreover, LF levels in PJ rose with the severity of CP. As PP in PJ was found in 55% of the patients with CP,but not in those with other diseases except for pancreatic cancer (8%), PP was considered to be relatively specific for CP. PP in PJ was observed more frequently in calcified CP (82%) than in non-calcified CP (55%). However, significant differences of abnormalires in pancreatogam and pancreatic function were no … More t observed between presence and absence of PP. Mean value and pevalence of abnormal LF in PJ in CP were higher in the PP positive group than in the PP-negative group. Concentration of CA 19-9 in PJ was significantly higher not only in pancreatic cancer (PC) but also in CP, and its prevalence was 92% in PC and 85% in CP. CA 19-9 in PJ in pj was considered to be a sensitive marker for non-specific pancreatic injury, but not for tumors. Concentrations of ST-439 and SLX in PJ were significantly increased only in PC, and their prevalences were 57% and 40%, respectively. They have high tumor-specificity, but their incidence was relatively low. CA 19-9 and CA-50, which are the type 1 chain carbohydrate antigens, were strongly expressed in pancreatic cancer, but were low in cancerspecificity. However, type 2 chain carbohydrate antigens such as CSLEX-1 and SLX showed highest tumor-secificity among the antigens, whereas their incidences were lower than other antigens. On the other hand, ST-439 and DU-PAN-2 exhibited high positivity and specificity. CA 19-9 was strongly and widely distributed mainly in the proliferative small pancreatic duct of CP. This may reflect the increased levels of CA 19-19 in PJ of patients with CP. Less