Study of Orthotopic Liver Transplantation in Warm Ischemically Damaged Graft-Protective Effects by Antioxidants-

1)再灌注损伤的现象不仅发生在短暂性肝缺血后，而且发生在肝移植过程中。用辅酶Q_<10>（CoQ&lt;@210）预处理30分钟热缺血损伤（30-WID）的供肝存活率（45%）明显高于未处理的供肝存活率（0%）。CoQ&lt;@D210@&gt;D2预处理完全抑制了30-WID肝移植后4 h脂质过氧化物的显著升高和内源性CoQ同系物（CoQ&lt;@D29@&gt;D2，Coq&lt;@D210@&gt;D2）的降低，从而改善了能量代谢。结果表明，外源性辅酶Q &lt;@D210@&gt;D2具有抗脂质过氧化作用。这些结果表明，大鼠原位肝移植后再灌注损伤的部分原因是刺激的生物膜脂质过氧化反应。2）肝移植的主要问题之一是如何在早期评估移植组织的活力。能力T ...更多信息 因此，评估移植物的即时功能将提供对确定预后有用的结果。在这项研究中，确定了肝移植后胆汁流速与三磷酸腺苷水平和接受移植的大鼠存活率相关。因此，在该实验移植模型中显示，移植后胆汁产生的监测是评估肝脏缺血性损伤程度和动物预后的有用指标。3）在肝移植手术中，袖带技术与手工缝合技术相比，（不缝合血管吻合）可以减少门静脉-肝动脉吻合过程中对肝脏的热缺血性损伤和血流动力学紊乱。静脉夹闭到受体，并且还避免由于凝血异常而出血。最近，日本开发了新的合成可吸收套囊材料（乳酸-乙醇酸共聚物作为可生物降解材料）。我们尝试将这种可吸收套囊材料应用于大鼠肝移植血管吻合。吻合后5个月，套囊层完全吸收，吻合血管腔内无任何狭窄和血栓形成。少

1) The phenomenon of reperfusion injury should occur not only after transient hepatic ischemia, but also during hepatic transplantation. Pretreatment with coenzyme Q_<10>(CoQ<@210)was accompanied by a siqnificant increase in the survival rete (45%) of 30-minute warm ischemically damaged (30-WID) liver grafts compared with 0% in nontreated liver grafts. CoQ<@D210@>D2 pretreatment completely suppressed the marked increase of lipid peroxide and decrease of endogenous CoQ homologues (CoQ<@D29@>D2, Coq<@D210@>D2) 4 hours after grafting of 30-WID liver, resulting in the improvement of enerfy metabolism. This result suggests that exogenous CoQ<@D210@>D2 acts as antioxidant of lipid peroxide. These findings provide that reperfusion damage in rat liver after orthotopic transplantaion is partly due to stimulated lipid peroxidation on biomembranes.2) One of the major problems accompanying liver transplantation is how to evaluate the viability of the grafted tissue at an early stage. The ability t … More o assess immediate graft function would provide results useful in the determination of prognosis. In this study, it was determined that bile flow rates after liver transplantation were correlated with adenosine triphosphate levels and the survival of rats given transplants. Thus it was shown in this experimental transplantation model that the monitoring of bile production after grafting is a useful indicator for assessing the extent of ischemic damage to the liver and for prognosis of the animal.3) In liver transplantation surgery, the cuff technique (non-suture vascular anastomosis) in comparison with hand-suture technique can minimize warm ischemic damage to the liver and hemodynamic diturbance during portal-venous clamping to the recipient, and also avoid hemorrhage due to coagulation abnormalities. Recently, new synthetic absorbable cuff materials (lactic acid-glycolic acid copolymer as a biodegradable material) have been developed in Japan. We attempted the application of this absorbable cuff materials to vascular anastomosis in rat liver grafting. The cuff layer was completely absorbed 5 months after anastomosis and any stenosis and thrombosis did not exit in the anastomosed-vascular lumen. Less