Correlation between concommitant immunity and postoperative recurrence in primary resected lung cancers

本研究的目的是证明对自体肿瘤细胞的细胞毒作用与肺癌术后复发的相关性。取原发灶标本培养自体肿瘤细胞，用Ficoll-Hypaqye不连续梯度分离外周血淋巴细胞，作为效应物检测术后细胞毒活性。1)对自体肿瘤细胞的细胞毒活性为-3.9-39.6%，平均为16.1%，对已建立的肺腺癌细胞系PC-3和白血病细胞系K562的平均细胞毒活性分别为29.6%和46.5%。针对这三个靶点的细胞毒活性在分期或可切除性方面没有统计学意义的变化，但在组织学类型之间有一些差异，即在腺…中对自体肿瘤细胞的细胞毒活性。2)分期评价腺癌对自体肿瘤细胞的杀伤活性，I期明显高于IV期(P&lt；0.05，t检验P&lt；0.05)，而鳞癌各期之间无显著差异。3)完整切除组，术后1~2周对自体肿瘤细胞的杀伤活性明显高于术后4~5周(P&lt；0.05，t检验)。4)术后4-5周对PC-3细胞或K562细胞的杀伤活性与术后1-2周相比有升高趋势，但不同分期、不同肺切除程度的患者术后4-5周对PC-3细胞或K562细胞的杀伤活性均较术后1-2周升高。较少

The objective of this study is to demonstrate the correlation between cytotoxic activity against autologous tumor cells and postoperative recurrence in primary resected lung cancers. Autologus tumor cells were cultured by the specimens oftained from primary sites and peripheral blood lymphocytes were separated by Ficoll-Hypaqye discontunuous gradient and utilized as effectors to measure postoperative cytotoxic activity. The rusults obtained herein are as follows:1) Overall cytotoxic activities against autologous tumor cells ranged from -3.9-39.6% with an average of 16.1%, and the average cytotoxic activities against established lung adenocarcinoma cell line PC-3 and leukemia cell line K562 were 29.6% and 46.5%, respectiyely. No statistically significant changes of cytotoxic activities against these 3 targets to stages or resectabilities were demonstrated, but there existed some differences between histologic types, that is, the cytotoxic activity against autologous tumor cells in adeno … More carcinoma tend to be higher than that of squamous cell carcinoma (P<0.1 by Student t test)2) When the cytotoxic activity against autologous tumor cells in adenocarcinoma was evaluated by stages, the value of stage I was significantly higher than that of stage IV(P<0.05 by Student t test), however, there were no significant changes between any stages in squamous cell carcinoma. Furthermore, no statistically significant differences between any stages in the cytotoxic activities against PC-3 or K562 targets of adenocarcinoma were demonstrated.3) In patients given a complete resection, the cytotoxic activity against the autologous tumor cells during the postoperative 1-2 weeks was significantly higher than during the postoperative 4-5 weeks (P<0.05, Student t test). In patients given an incomplete resection, however, no significant difference was demonstrated between the postoperative 1-2 weeks and during the postoperative 4-5 weeks.4) In contrast, the cytotoxic activity against the PC-3 cells or K562 cells during the postoperative 4-5 weeks tended to rise in comparison to those at the postoperative 1-2 weeks all patients in any cancer stage and whatever the amount of their lung resection.From these rusults, we conclude that there may exist a concommitant immunity in primary lung cancers, and that ATCA appears to be a good prognostic parameter in lung cancers, particularly adenocarcinomas. Less