Production of useful tumor markers for gyvecologic tumors.

制备了一种抗人子宫内膜腺癌细胞系的小鼠单克隆抗体MCA-97。在细胞酶联免疫特异性实验中，MCA-97抗体与所有腺癌细胞系反应，包括四种子宫内膜腺癌细胞系。估计MCA-97定义的抗原是一种非唾液化的高分子量糖蛋白，其行列式中含有半乳糖和n -乙酰氨基葡萄糖。在福尔马林固定、石蜡包埋切片的免疫过氧化物酶染色中，MCA-97与大多数子宫内膜腺癌和卵巢子宫内膜型腺癌反应，也与女性生殖器和胃肠道的正常腺上皮反应。然而，它在方状细胞癌和卵巢浆液性腺癌组织中大多无反应。通过反向被动血凝法，在三分之一的子宫内膜样腺癌患者和一半的卵巢子宫内膜腺癌患者的血清中检测到MCA-97定义的抗原。该抗原在大多数正常女性志愿者血清中不可见。因此，MCA-97在血清学和病理学诊断方面具有潜在的临床应用价值。

A murine monoclonal antibody ,MCA-97,was prepared against a human endometrial adenocarcinoma cell line. In cellular enzyme-linked immunospecific assay,the MCA-97 antibody reacted with all adenocarcinoma cell lines tested including four endometrial adenocarcinoma lines. The antigen defined by MCA-97 was estimated to be a non sialylated high-molecular-weight glycoprotein containing galactose and N-acetylglucosamine in its determinant. In immunoperoxidase staining of formalin-fixed, paraffin-embedded sections,MCA-97 reacted with most endometrial adenocarcinomas and ovarian endometrial-type adenocarcinomas, and also reacted with normal glandular epithelium of the female genital and gastrointestimal tracts. However, it was mostly unreactive with squarous cell carcinoma and ovarian serous adenocarcinoma tissues. By the reversed passive hemagglutination method, the antigen defined by MCA-97 was detected in the sera of one-third of patients with endometrioid adenocarcinomas, and in half of those with ovarian endometrial adenocarcinamas. The Antigen was not demonstrable in sera of most normal female volunteers. Thus, MCA-97 has potential clinical applications in diagnostic serology and pathology.