Behavior of Langerhans Cells in the Hemopoietic Tissue, Skin, and Lymph Nodes.

朗格汉斯细胞在皮肤相关免疫系统中执行抗原提呈功能。研究了朗格汉斯细胞在小鼠皮肤、口腔粘膜、前胃和淋巴结中的行为。采用atp酶组织化学和免疫组织化学方法对表皮和粘膜上皮片进行Ia抗原染色，以证实朗格汉斯细胞。电镜检查伯贝克颗粒的出现情况。研究了朗格汉斯细胞与thy -l阳性细胞在前胃表皮和上皮中的分布关系。研究了禽皮肤表皮中atp酶阳性朗格汉斯样细胞的发生。这些研究结果总结如下：1)未成熟朗格汉斯细胞在胎儿时期进入表皮。这些细胞呈atp酶阳性，但Ia抗原阴性。2)出生后1周内，这些细胞在细胞膜上表达Ia抗原，胞质内形成Birbeck颗粒。出生后一周，它们的数量增加到成人皮肤的频率水平。3)然后它们开始通过淋巴管到达局部淋巴结，到达皮质旁区，在那里它们与淋巴细胞或指间细胞（ID）密切接触。4)在3周龄前，粘膜中有少量朗格汉斯细胞。此后，它们的数量开始增加。5)在前胃粘膜上，朗格汉斯细胞在上皮细胞之间延伸出很长的树突，因此每个细胞占据很大的空间。6)朗格汉斯细胞与thy -l阳性细胞有密切的分布关系。7)在禽表皮中发现了atp酶阳性的朗格汉斯样细胞。

Langerhans cells perform an antigen presenting function in the skin-associated immune system. The behavior of the Langerhans cells was studied in the mouse skin, oral mucous membrane, forestomach, and lymph nodes. To demonstrate the Langerhans cells, epidermal and mucosal epithelial sheets were stained by ATPase histochemistry and immunohistochemical methods for Ia antigens. The occurrence of Birbeck granuls was examined by electron microscopy. The distributional relationship between the Langerhans cells and Thy-l-positive cells was investigated in the epidermis and the epithelium of forestomach. The occurrence of ATPase-positive, Langerhans-like cells was investigated in the epidermis of avian skin. The results of these studies were summarized as follows : 1) Immature Langerhans cells enter the epidermis during the fetal period. These cells are ATPase-positive, but Ia antigen negative. 2) Within 1 week after birth, these cells express Ia antigen on their cell membrane and form Birbeck granules in their cytoplasm. They increase in number to the level of the frequency of adult skin by 1 week after birth. 3) They then begin to travel to the regional lymph node via lymphatic vessells, and reach the paracortical area, where they make close contacts with lymphocytes or Inter=Digitating (ID) cells. 4) In the mucous membrane Langerhans cells occur in small numbers before 3 weeks of age. They begin to increase in number thereafter. 5) In the mucous membrane of forestomach, Langerhans cells extend long dendritic processes between the epithelial cells, thus each cell occupies a large space. 6) There is an intimate distributional relationship between Langerhans cells and Thy-l-positive cells. 7)In the avian epidermis ATPase-positive Langerhans-like cells were discovered in the present study.