Virological and immunological study on non-A, non-B hepatitis

我们从非甲、非乙型肝炎患者的肝细胞和淋巴细胞原代培养物中检测病毒。然而，到目前为止，我们还没有找到任何负责任的代理人通过这种方法。1989年Choo等和Kue等在Chiron公司的研究组中，成功地分离了丙型肝炎病毒（HCV）的基因产物，建立了HCV循环抗体的检测系统。使用Ortho诊断系统公司提供的ELISA试剂盒，检测了非A、非B型急、慢性肝病患者血清中抗-HCV抗体。抗-HCV抗体在急性散发性非甲非乙型肝炎、输血后非甲非乙型肝炎、慢性非甲非乙型肝炎、肝硬化和非甲非乙型肝癌中的阳性率分别为44%、62%、76%、64%和70%。而在B肝炎和肝硬化患者中，这种抗体的检出率低于5%。抗-HCV阳性者慢性化倾向明显高于抗-HCV阴性者。15例慢性肝炎和输血后肝硬化患者抗-HCV均阳性，提示HCV持续感染与肝癌的发生密切相关。与慢性B型肝炎相比，血清β 2微球蛋白水平升高程度较轻，肝内HLA 1类抗原表达较弱。但与慢性B型肝炎相比，干扰素治疗后小叶内浸润淋巴细胞的下降程度更明显，提示干扰素对非甲、非B型肝炎和B型肝炎的疗效存在差异。

We have tried to detect the virus(es) in the hepatocytes arid lymphocytes of primary culture after being obtained from patients with non-A,non-B hepatitis. However, until now we could not find any responsible agent by this method. In 1989, Choo et al and Kue et al in Chiron Co. research group diicceeded to isolate the gene product of hepatitis C virus (HCV) and established an assay system for circulating antibody to HCV. Using the ELISA test kit provided by Ortho Diagnostic System Co., iwe tested anti-HCV antibody in sera of patients with acute and chronic liver diseases of non-A,non-B type. Anti-HCV antibody vas positive in 44 % of acute sporadic non-A,non-B hepatitis, in 62 % of posttransfusion non-A,non-B hepatitis, in 76 % of chronic hepatitis of non-A,non-B type, in 64 %"of liver cirrhosis and 70 % of hepatocellular carcinoma of non-A,non-B type respectively: To the contrary, in type B hepatitis and cirrhosis, this antibody vas detected only in less than 5 %.Among the patients with non-A,non-B acute hepatitis, anti-HCV positive cases had a significantly higher tendency to chronicity as compared with anti-HCV negative cases. All 15 cases of chronic hepatitis and cirrhosis of,.posttransfusion etiology who developed finally hepatocellular carcinoma were positive for anti-HCV, indicating that there is a intimate relationship between persistent HCV infection and development of hepatocellular carcinoma.Regarding to the response to interferon administration in non-A,non-B chronic hepatitis, serum beta 2 microglobulin level was much less elevated and intrahepatic expression of HLA class 1 antigen was much weaker as compared with those in chronic hepatitis type B. However, intralobular infiltrating lymphocytes decreased much more significantly in grade after interferon administration as compared to that in chronic hepatitis of type B, suggesting that there are differences of therapeutic responses to interferon between non-A,non-B hepatitis and type B hepatitis.