The role of VLA proteins and fibronectin in the activation of synovial cells from rheumatoid arthritis.

VLA蛋白和纤维连接蛋白在类风湿关节炎滑膜细胞活化中的作用。在这项研究中，我们的目的是阐明VLA与类风湿关节炎患者滑膜细胞的相互作用，以及配体FN在类风湿关节炎滑膜炎发病机制中的作用。首先，我们检测了滑膜组织中VLA蛋白(VLA2、3、4、5)的表达。滑膜衬里和滑膜下层细胞表达VLA3、VLA4和VLA5蛋白。淋巴细胞主要表达VLA-4。血管内皮细胞表达VLA-2、VLA-3和VLA-5蛋白，比较外周血T细胞、滑膜T细胞和滑液T细胞VLA-4蛋白的表达，发现滑液T细胞上VLA-4表达增加，提示滑液中存在VLA-4诱导物质。接下来，我们研究了纤维连接蛋白及其片段是否影响滑膜细胞的增殖。虽然他们没有这样做，但他们诱导了滑膜A细胞的形状变化。当我们检测到纤维连接蛋白刺激后的滑膜细胞中的磷酸化蛋白时，我们没有得到恒定的模式。最近报道，血管细胞黏附分子-1(VCAM-1)是VLA-4蛋白的另一种配体。因此，我们检测了VCAM-1在类风湿关节炎患者滑膜组织中的表达。这种表达出现在滑膜衬里和下层细胞上。由于这些细胞大多表达VLA-4蛋白，这些细胞之间的相互作用可能归因于VLA-4和VCAM-1分子的结合。

Title : The role of VLA proteins and fibronectin in the activation of synovial cells from rheumatoid arthritis.In this study, our aim was to clarify whether the interaction of the very late antigen (VLA) on synovial cells from rheumatoid arthritis patients and that ligand, fibronectin is involvd in the pathogenesis of rheumatoid arthritis synovitis. First, we examined the expression of VLA proteins (VLA2, 3, 4, 5) on synovial tissues. VLA3, 4 and 5 proteins were expressed on the synovial lining and sublining cells. Lymphocytes mainly expressed VLA-4. Endothelial cells expressed VLA2, 3 and 5 proteins.When we compared the expression of VLA-4 protein among peripheral blood T cells, synovial T cells and synovial fluid T cells, we found the increased expression of VLA-4 on synovial fluid T cells, suggesting the existence of VLA-4 inducing substance in synovial fluid. Next, we examined whether fibronectin and that fragments affect the proliferation of synovial cells. Although they did not so, they induced the shape change of synovial A cells. When we detected the phosphorylated proteins in synovial cells after the stimulation of fibronectin, we did not get the constant pattern. Recently it was reported that the vascular cell adhesion molecule-1 (VCAM-1) was the other ligand of VLA-4 protein. Thereby, we examined the expression of VCAM-1 in the synovial tissues from rheumatoid arthritis patients. That expression was shown on the synovial lining and sublining cells. Since most of these cells express the VLA-4 protein, the interaction of these cells might be attributed to the binding of VLA-4 and VCAM-1 molecules.