The development of Fruit Tree cultivar identification using DNA fingerprinting

本研究旨在探索DNA指纹技术在果树品种鉴定中的新应用。首先，对5种已知的人类DNA指纹探针与柑橘DNA的杂交能力进行了检测。结果表明，克隆的牛噬菌体M13小卫星样结构域可用于柑橘品种的鉴定。其次，利用DNA指纹技术对柑橘品种进行鉴定。我们使用基于PCR技术的小卫星序列检测DNA多态性。为了这项研究，根据M13小卫星序列合成了四条引物。结果表明，两对引物组合能有效地扩增出多态性片段，并将扩增产物连接到质粒载体上进行DNA指纹图谱分析，其中部分片段含有柑橘类小卫星序列，为缺乏DNA序列信息的果树品种鉴定提供了一个很好的工具。

This study is an attempt to develop the new application of fruit tree cultivar identification using DNA fingerprinting.At first, five probes known to allow DNA fingerprinting in humans were checked for their ability to hybridize to Citrus DNA.The results showed that the clone of M13 phage minisatellite-like domain of bovine DNA was useful for the identification of Citrus cultivars.Secondly, we detected DNA polymorphisms with the PCR-based technique using minisatellite sequence. For this study, four primers were synthesized on the basis of M13 minisatellite sequence. The results showed that polymorphic fragments were amplified effectively by the combination of two primers.In addition, PCR products were ligated into the plasmid vector and used for DNA fingerprinting because some of them contained Citrus minisatellite-like sequences.We suggest that the strategy in this study is a good tool for cultivar identification of fruit tree which have poor information on DNA sequence.