Structural Analysis of Sulfated Polysaccharide with Anti-AIDS Virus.

海洋假单胞菌(HA-318)产生一种含有岩藻糖胺的多糖。用1%的苯酚溶液提取多糖，加入乙醇，用Cetevlon沉淀和DEAE-纤维素柱层析进行纯化。该多糖的组成为半乳糖醛酸、半乳糖、岩藻糖胺、丙酮酸，摩尔比为3：1：1：1，并含有N-乙酰基。以海洋假单胞菌中含有岩藻糖胺的多糖为原料，经氯磺酸处理后得到的多糖的磺化衍生物对单纯疱疹病毒1型具有较强的抗病毒活性。该硫酸多糖在0.72ug/ml的浓度下可抑制HSV-1的细胞病变作用。当浓度达到1000ug/ml时，对Vero细胞无细胞毒作用。用0.6M氯化钠洗脱的部分显示出较高的抗HIV-1活性(IC_&lt；50&gt；=0.69 ug/ml)，高于葡聚糖硫酸盐(1.5 ug/ml)。当浓度达到100ug/ml时，该样品对MT-4细胞无细胞毒性。值得注意的是，在硫酸盐化程度(S=5.6%，W/W)低于硫酸葡聚糖(S和GT；17%，W/W)的情况下，该样品具有抗HIV-1作用。

A marine strain of Pseudomonas (HA-318) produces a polysaccharide with a rate component, fucosamine. The polysaccharide was extracted with 1% phenol solution and purified by addition of ethanool followed by precipitation with Cetevlon and DEAE-cellulose eolumn chromatography. This polysaccharide preparation, which was homogeneous in both ultracentrifugal and electrophoretic analyzes, was composed of galacturonic acid, galactose, fucosamine, and pyruvate in the molar rations of 3 : 1 : 1 : 1, together with N-acetyl groups.Sulfated derivatives of the polysaccharide prepared by chlorosulfonic acid treatment of a fucosamine-containing polysaccharide from a marine Pseudomonas showed strong anti-viral activity ageinst herpes simplex type 1 (HSV-1). This sulfated polysaccharide inhibited the cytopathic effect of HSV-1 at 0.72 ug/ml. No cytotoxic effects to Vero cells were detected with concentrations of sulfated polysaccharide up to 1,000 ug/ml.Sulfated derivatives was fractionated by chromatography on a DEAE-celulose column. The fraction eluted with 0.6M NaCl showed the anti-HIV-1 activity (IC_<50>=0.69 ug/ml) which was higher than that of dextran sulfate(1.5 ug/ml). No cytotoxicity of this sample for MT-4 cells was noted at concentration up to 100 ug/ml. It should be noted that this showed an anti-HIV-1 effect at a lower degree of sulfation (S=5.6%, W/W) than dextran sulfate (S >17%, W/W).