Effect of EDLF on Ca^<2+>, Na^+ pump in cultured vascular smooth muscle cells

近年来对内皮细胞和血管平滑肌细胞（VSMC）相互作用的研究揭示了内皮细胞和血管平滑肌细胞之间利用体液因子的相互调节机制。这些调节的体液因子包括内皮素、EDRF和前列腺素。在此背景下，目前的研究主要集中在这两个细胞之间的相互关系上。为此，我评估了内皮源性激素（ET-1）对VSMC阳离子运输的影响。此外，我尝试了一种新的培养体系，VSMC与内皮细胞共培养。第一部分研究表明，ET-1刺激Na-K泵活性、Na-H反端口和Na-K转运。ET-1本身也刺激钙内流，导致VSMC胞质游离钙浓度增加。考虑到氮基药物可以模拟EDRF的作用，本研究的第二部分描述了氮基药物对VSMC阳离子转运系统的影响，表明NO对VSMC的增殖有抑制作用。由于耗时的技术困难，共培养实验未能在2年的研究期限内完成，无果而终。

Recent investigations of the interaction between endothelial cells and vascular smooth muscle clls(VSMC) have disclosed mutual regulatory mechanism, utilizing humoral factors, between the two cells.Such regulatory humoral factors include endothelin, EDRF and prostaglandins.Present study has, in this context, focused on the interrelationship between the two cells. To pursue this, I assessed the effect of endothlium-derived hormone(ET-1) on cation transport of VSMC.In addition, I attempted a new culture system, co-culture of VSMC and endothelial cells. The 1st part of the study showed that ET-1 stimulates Na-K pump activity, Na-H antiport and Na-K contransport. ET-1, per se, also stimulates Ca influx, resulting in an increase in cytosolic free Ca concentration of VSMC.Knowing that a nitro-based agent may mimic the effect of EDRF, the 2nd part of this study described the effect of nitro-based pharmacological agent on cation transport s stems of VSMC, which showed an inhibitory action of NO on the proliferation of VSMC.Because of time-c nsuming technical difficulties, the co-culture experiment could not be completed within the study's 2-year limit and thus was terminated without results.