Study on mechanisms of intravenous anesthetic agents by ^1H-NMR

为了弄清静脉麻醉药的作用机理，我们通过测量生物高分子到水的自旋-晶格弛豫时间（T_1）和分子间交叉弛豫时间（T_2），研究了麻醉药在组织中的水结构<IS>。这一点非常重要，生物样品上的^1H-NMR实验的主要部分是使用内径为1.1 mm的玻璃毛细管进行的，该玻璃毛细管插入到含有少量溶剂的5 mmphi NMR管中用于锁定，具有带有中心孔的小棒状塑料垫片。方法（A）、（B）、（C）是由藤田保健大学综合医学研究所分子生理学研究室使用大鼠的肝或脑组织进行的基础研究。方法（A）：将内径为1.1mm的两端开口的NMR玻璃毛细管转向大鼠肝脏或大脑，将组织进入毛细管一端后，用塑料糊将毛细管两端封闭。 ...更多信息 将组织侧糊剂用瞬时粘合剂覆盖后，插入充满D_2O的5 mmphi NMR管的大部分中心孔内锁紧。方法（B）：安排方法（A），用内径1.1mm的一端封闭的玻璃毛细管，用75 μ l微量移液器穿入组织，用塑料糊封闭开口，插入装有二甲基亚砜-d_6的5 mmphi NMR管中，按方法（A）进行锁定。方法（C）：根据方法（B）将内径为1.1 mm的NMR玻璃毛细管插入到用于NMR的专用试管中;内径为3.2 mm，外径为3.98 mm，该试管插入到具有少量二甲基亚砜-d_6的5 mm phi NMR管中。对（A）、（B）、（C）三种方法制备的生物样品，用Brucker AM 500核磁共振谱仪研究了匀场控制和T_1、T_2重定值的难易程度<IS>。结果表明，匀场控制非常容易，并证明我们可以测量T_1和T_2的<IS>方法（C）。少

We studied on water structure in the tissues for anesthetic agents by measuring spin-lattice relaxation times (T_1) and intermolecular cross-relaxation times (T_<IS>) from biopolymer to water, as a result of making clear for mechanisms of intravenous anesthetic agents. This is very important that the major parts of ^1H-NMR experiments on biospecimens are carried out using glass capillaries of 1.1mm inner diameter, inserted into a 5mmphi NMR tube containing a small volume of solvent for locking, with small rod-shaped plastic spacers with a center hole. We conceived the methods (A), (B) and (C), included basic studies using liver or cerebral tissues of rats with department of molecular physiology, comprehensive medical institute of Fujita Health University. Method (A) : NMR glass capillaries of 1.1mm inner diameter with both ends open were pierced as turning to a rat liver or cerebrum, closed up the both ends of the capillaries by plastic paste after the tissues got into one end of the c … More apillaries, and inserted into the center hole for the most part of a 5mmphi NMR tube filled up D_2O for locking after covered the tissue side paste by an instantaneous adhesive agent. Method (B) : Arranged the method (A), carried out using one end closed glass capillaries of 1.1mm inner diameter after pierced a 75mul micropipette to the tissue, closed up the open side by plastic paste, and inserted to a 5mmphi NMR tube filled up dimethylsulfoxide-d_6 for locking according to the method (A). Method (C) : After inserted NMR glass capillaries of 1.1mm inner diameter according to method (B) to a special test tube for NMR ; 3.2mm inner & 3.98mm outside diameter, that was inserted to a 5mmphi NMR tube with a small quantity of dimethylsulfoxide-d_6. For the biospecimens that made by the methods (A), (B) and (C), we studied degree of difficulties to shimming control and reappering of T_1 and T_<IS> by Brucker AM500 NMR spectrometer. In results, shimming control was very readily, and turned out as we could measuring of T_1 and T_<IS> for the mothod (C). Less