Distribution and forms of fluoride in normal fuman plasma

本研究的目的是用凝胶色谱法测定人血浆中氟化物的基本范围和平均浓度。在低氟化物饮食饲养的大鼠中，每天通过胃插管给药一次有机氟化物（DF）和氟化钠，测量了大鼠器官中的氟化物水平。实验结果如下：1.实验结果表明：在一项使用市售人血清的polot研究中，测定了三种类型的氟化物（离子型、非离子型结合型和非离子型低分子型），结果与以往关于动物血浆和血清的数据相似^<*\)>。因此，凝胶色谱法是研究血浆和血清中三种氟化物的有效方法。然而，凝胶色谱法对志愿者血浆和血清的研究并不成功，因为血浆中的大部分非离子型氟化物是结合氟化物，导致结合氟化物水平远高于非离子型氟化物水平。这些事实表明，凝胶色谱法必须进一步研究不同动物类型和血浆和血清成分。有机氟化物（DF）剂量组未引起血浆中氟离子升高。然而，氟化钠剂量组表现为血浆中氟离子浓度升高，硬组织中氟离子浓度与血浆中氟离子水平有关，因为硬组织中氟离子浓度在血浆中表现为水平，因为硬组织中氟离子浓度在血浆中表现为类似的趋势。低氟饮食可用于动物实验中血浆和其他组织中不同微量氟化物的研究。^<* * * * * * * * * * * * * * * * * * * * * * * * * * * *卫生，40,456-457,1990。(在日本)

The objective of this research was to determine by the gel chromatography method the basal range and the average concentration of fluoride in human plasma. The fluoride levels in the rat organs were measured when the organic fluoride compound (DF) and sodium fluoride were administered by gastric intubation once a day while breeding on a low fluoride diet.The results were as follows :1.In a polot study using market human serum, three types of fluoride (ionic, non-ionic bound and non-ionic low molecular) were measured, the results were similar to those of previous data concerning plasma and serum of animals^<*\)>. Therefore, the gelchromatography method proved to be an effective technique for the study of the three types of fluoride in plasma and serum.2.However, the gel chromatography method was not successful for the study of the plasma and serum taken from volunteers because most of the non-ionic fluoride in the plasma was bound fluoride resulting in a bound fluoride level much higher than the non-ionic fluoride level. These facts suggest that the gel chromatography method must be investigated further with various animal types and cmponents of plasma and serum.3.The organic fluoride compound (DF) dose group did not result in an increase of ionic fluoride in the plasma. However, the sodium fluoride dose group shwed an increase of ionic fluoride in the plasma and the hard tissues is related to the ionic fluoride level in plasma because the ionic fluoride cancentration in hard tissues showed a lebel in plasma because the ionic fluoride concentration in hard tissuses showed a similar tendency in the plasma.4.The low fluoride diet was useful for the study of different trace amounts of fluoride of plasma and other tissues in animal experiments.^<*\)>Koyama M., et al. : A study on fluoride in plasma protein by gel chromatography, J.Dent. Health, 40, 456-457, 1990. (in japanese)