SIMULTANEOUS QUANTITAION OF INSULIN AND IT'S RELATED 12 PEPTIDES IN BLOOD

研究了用离子喷雾质谱结合高压液相色谱法分析肽类激素，用于同时定量胰岛素及其相关的生物活性肽类激素：胰高血糖素、分泌素、β-内啡肽、生长抑素、血管紧张素I、II和III、胃泌素释放肽、心房利尿肽、促肾上腺皮质激素、胃抑制多肽和血管活性肠多肽。在相应分析物的基峰处进行选择离子监测（SIM）。所有峰在35分钟内分离。13种多肽混合物的各组分的同时LC/MS给出了在14 fmol/ mu ~（-1）至6 pmol/ mu ~（-1）浓度范围内具有令人满意的线性的校准曲线。还尝试了使用Mitrix辅助激光解吸（MALD）电离结合飞行时间（TOF）来获得更高的灵敏度。质谱仪，并获得了5 fmol的灵敏度。进一步的调查定量肽激素在血液中的进展。

Analyzes of peptide hormones by ion spray mass spectrometry coupled with high-pressure liquid chromatography have been studied for the simultaneous quantitations of insulin and it's related biologically active peptide hormones ; glucagon, secretin, beta-endorphin, somatostatin, angiotensin I, II, and III, gastrin releasing peptide, atriuretic peptide, adrenocorticotropic hormone, gastric inhibitory polypeptide, and vasoactive intestinal polypeptide. Selected ion monitoring (SIM) was made at the base peaks of the corresponding analytes. All peaks were separated within 35 min. The simultaneous LC/MS of each component of the mixture of 13 peptides gave calibrationcurves with satisfactory linearity over the concentration range of 14 fmol/ mu 1 to 6 pmol/ mu 1.Attempts were also made to obtain higher sensitivity by the use of mitrix-assisted laser desorption (MALD) ionization coupled with time of flight (TOF) mass spectrometer and obtained the sensitivity of 5 fmol.Further investigations for the quantitation of peptide hormones in blood are in progress.