Sterile effect of benzoylphenylurea type insect growth regulator

苯甲酰苯基脲类化合物对昆虫具有独特的生理效应。当用定虫隆（CF）（BPU化合物之一）处理昆虫幼虫时，昆虫在蜕皮阶段被杀死。CF处理对产卵也有不育作用。雄性幼虫处理后，这种影响更为严重。本试验主要以斜纹夜蛾为试验材料。(1)CF对斜纹夜蛾幼虫的不育作用对雄虫的影响大于雌虫。在小菜蛾中也观察到同样的现象。(2)用CF处理5龄幼虫，在6龄3日龄以后，睾丸的大小明显小于对照。(3)CF处理5龄幼虫后，蛹和成虫的无核和真核精子束数量均减少。CF处理还能延缓精子从睾丸向输精管的运动。(4)CF处理不影响交配行为。但转移到雌虫体内的无核精子束数量减少。无核期精子束数与所产卵的不育效应呈高度相关。(5)CF处理影响睾丸蜕皮激素滴度。CF的不育作用可能与其降低蜕皮激素的滴度有关。CF对蜕皮激素滴度的影响有待进一步研究。通过这类工作有可能解决BPU抑制几丁质合成的作用方式。

Benzoylphenylurea (BPU) compounds show a unique physiological effect to insects. When insect larvae are treated with chlorfluazuron (CF) (One of BPU compounds), insects are killed at molting stages. CF treatment also showed a sterile effect in the oviposited eggs. This effect was more serious when male larvae wee treated. Spodoptera litura was mainly used in this experiment. (1) When larvae of S.litura were treated with CF, sterility effect was observed more seriously in males than females. The same phenomenon was also observed in Plutella xylostella. (2) When 5th instar larvae wee treated with CF, the size of testis was observed to be smaller in CF treated larvae than those of the control ones after stages of 3rd day of 6the instar. (3) When 5th instar larvae were treated with CF, the numbers of apyrene and eupyrene sperm bundles were smaller in treated pupae and adults. The movements of sperm from testis to vas diference was also delayd by CF treatment. (4) CF treatment did not affect mating behavior. However, the number of apyrene sperm bundles transferred to females was decreased. There was a high correlationship between the number of apyrene sperm bundle and sterility effect of oviposited eggs. (5) CF treatment affected the amount of ecdisteroid titer of testis. The sterility effect of CF was thought to be involved in the decrease of ecdisteroid titer. Further studies how CF affect ecdisteroid titer should be performed. There might be a possibility that the mode of action of BPU on the chitin synthesis inhibition will be solved through this kind of work.