BIOLOGICAL ASPECTS OF RESTING EGG FORMATION OF MARINE ROTIFERS AND ITS APPLICATION TO FINFISH HATCHERIES

对不同种群间交配行为的观察表明，褶皱B.plicatilis与圆形B.rotundiformis之间以及B.plicatilis与SS品系之间存在生殖隔离。相反，在圆型B.rotundiformis和SS菌株之间观察到交配。因此，没有证据表明SS-菌株是一个新的海洋臂尾轮虫种，而更有可能被归入B型臂尾轮虫中。采用半连续培养方法可以提高产休眠卵的效率。一些半连续培养物在第15- 20天由于细菌效应而崩溃。然而，通过放置过滤垫以去除有机材料来稳定轮虫培养物。因此，获得了8.1x10^2个休眠卵/天/g（干重）的收获，这比分批培养的效率高3.0倍。然而，在更换培养水后，圆形B. roundiformis的休眠卵没有大量产生。

Observations on mating behavior between different groups indicated that there is reproductive isolation between B.plicatilis and B.rotundiformis and between B.plicatilis and SS strains. In contrast, copulation was observed between B.rotundiformis and SS-strains. Thus, there is no evidence that SS-strains are a new marine Brachionus species, and most likely should be included in B.rotundiformis.The efficiency of resting egg production can be improved by employing the semi-continuous culture method. Some semi-continuous cultures collapsed on 15-20th days due to the bacterial effect. However, the rotifer culture was stabilized by placing filtration mats to remove organic materials. Thus harvest of 8.1x10^2 resting eggs/day/g (dry weight) Nannochloropsis oculata was achieved, which is 3.0 times more efficient than from batch cultures. Resting eggs of B.rotundiformis, however, were not mass produced after the exchange of culture water.