Forensic Neuropathological Study of Fibroblast Growth Factor and Stress Protein

1.应用免疫组化方法对36例法医尸检标本进行了热休克蛋白、HSP 72和泛素的检测，包括窒息、创伤、SIDS、中毒及其他。在窒息、酒精、甲苯和药物中毒时，海马神经细胞胞浆内可见HSP 72的表达。这些结果表明，细胞和组织中的HSP反映了作为死亡原因的损伤。在法医学尸检中，HSP的检测对了解死亡原因和机制具有重要意义采用免疫组化法检测HSP和FGF的表达，结果发现，缺氧缺血性脑损伤后存活2- 5 h的病例，残存神经元呈a-FGF和b-FGF免疫反应，但胶质反应不明显。存活时间较长的病例可见神经元坏死和丢失，并伴有胶质细胞酸性蛋白（GFAP）、波形蛋白（vimentin）和a-FGF阳性星形胶质细胞增生。3.对18例闭合性颅脑损伤伴局灶性损伤的病例进行弥漫性轴索损伤（DAI）病理检查。11例（61.1%）可见轴索和髓鞘变性及回缩球。为了解DAI时神经元的变化，对这些病例的海马进行了检查。免疫组化显示MAP、EK、VIP、HSP、FOS和FGF在海马CA 2-CA 4区表达。但在CA 1区，这些染色减弱。因此，CA 1区锥体细胞不仅表现出形态学的改变，而且还表现出功能的退化。另一方面，CA 2-CA 4区锥体细胞具有一定的保护和修复机制，因为VIP、HSP 72、c-FOS和b-FGF被认为对神经细胞的变性具有保护和修复作用。

1.Immunohistochemically, heat shock proteins, HSP72 and ubiquitin, were demonstrated in 36 cases of forensic autopsy : asphyxia cases, trauma cases, SIDS,intoxication and others. In cases of asphyxia, alcohol-, tolen-and drug-intoxication, HSP72 could be observed in the cytoplasm of nerve cells of hippocampus. These results suggest that HSP in cells and tissues reflect injuries as the cause of death. In forensic autopsy, detection of HSP in affected organs is considered to be very useful for understanding the cause and mechanism of death.2.A neuropathological study of 47 forensic autopsy cases (hypoxic/ischemic brain damage) has been undertaken, using immunohistochemical staining to detect the HSP and FGF.In cases surviving 2-5h after hypoxic/ischemic injury, ischemic changes were seen, and the remained neurons showed immunoactivitiy of a-FGF and b-FGF.But glial reactions were not apparent. In cases of longer survival, neuronal necrosis and loss of neurons were seen, and these changes were accompanied by proliferation of glial fibrillary acidic protein (GFAP) , vimentin-positive and a-FGF-positive astrocytes.3.Neuropathologically, pathological changes of diffuse axonal injury (DAI) was examined in 18 cases of closed head unjury with local injuries. In 11 cases (61.1%) , axonal and myeline degeneration and retraction balls could be observed. To make clear the neuronal changes in DAI,hippocampus of those cases was examined. Immunohistochemistrically, MAP,EK,VIP,HSP,FOS and FGF were observed in CA2-CA4 area in hippocampus. But in CA1 area those stainabilities were decreased. Therefore, pyramidal cells in CA1 shows not only morphological change but also functional degenerations. On the other hand, pyramidal cells in CA2-CA4 have some protecting and repairing mechanism because it is considered that VIP,HSP72, c-FOS and b-FGF have effects to protect and repair against the degenerations of nerve cells.