USEFULNESS OF LIQUID VENTILATION BY PERFLUOROCARBON IN EXEPIMENTAL MODEL OF ACUTELUNG INJURY

我们研究了使用全氟化碳（Liqui Vent ; LV）的液体通气对体重10-15 kg的麻醉绵羊由内毒素血症诱导的急性肺损伤的影响。我们事先准备了肺淋巴瘘和血流动力学监测。腹腔注射大肠杆菌内毒素10 μ g/kg。v.通过气管插管将LV滴入肺后1小时。实验方案：（1）麻醉条件：FiO_2 0.5，TV 12 ml/kg，RR 30/min，PEEP 5cmH_2O LV，10 ml/kg;（2）麻醉条件：（1）肺动脉压（PaO_2）0.5，呼吸频率（RR）30/min，肺动脉压（PaO_2）10 ml/kg;将绵羊分为内毒素组和内毒素+LV组，每组10只，每只绵羊的FiO 2为1.0，TV为12 ml/kg，RR为30/min，PEEP为5cmH 2 O，LV为20 ml/kg。在实验（1）中，LV滴入肺内预处理可部分抑制内毒素引起的肺动脉高压、肺淋巴流量增加、低氧血症和外周血白细胞减少。实验（2）中，内毒素引起的上述变化不受左室灌注的抑制，实验（1）和（2）中FiO 2和左室容积的不同可能导致实验（1）和（2）的结果不同。全氟化碳液体通气对急性肺损伤的影响有待进一步研究。

We investigated the effects of liquid ventilation using perfluorocarbon (Liqui Vent ; LV) on acute lung injury induced by endotoxemia in anesthetized sheep weighing 10-15 kg. We had previously prepared the lung lymph fistula and hemodynamic monitering. E.coli endotoxin of 10 ug/kg was infused i. v. 1 hr after LV instillation into the lungs through the trachea tube. Anesthesia was continued by intravenous pentobarbital sodium of 25 mg/kg and panclonium of 0.1 mg/kg.Measurements : Pulmonary hemodynamics, systemic hemodynamics, lung lymph balance, arterial blood gas analysis, peripheral leukocyte countsExperimental protocol :(1)Anesthesia condition ; Fio2 0.5, TV 12ml/kg, RR 30/min, PEEP 5cmH2O LV ; 10 mi/kg(2)Anesthesia condition ; FiO2 1.0, TV 12ml/kg, RR 30/min, PEEP 5cmH2O LV ; 20 ml/kgIn each experiment sheep were divided into the endotoxin alone and the endotoxin plus LV group.Results are as follows. In experiment (1) , pulmonary hypertension, increased lung lymph flow, hypoxemia and peripheral leukopenia induced by endotoxin were partially suppressed by pretreatment of LV instillation into the lungs. However in experiment (2) these changes induced by endotoxin were not suppressed by LV instillation.The differences of FiO2 and the volume of LV might produce the different results between experimental (1) and (2) . We need further studies to see the effects of the liquid ventilation by perfluorocarbon on acute lung injury.