Apolipoprotein Phenotypes in Healthy Controls and Demented Patients from the Inhabitants in the middle and Northern mie Prefecture of Japan

为探讨载脂蛋白E4(Apo E4)与阿尔茨海默病(AD)发病的关系，我们分析了日本三叶县中北部地区692名健康人和75例痴呆患者的载脂蛋白E(Apo E)表型分布，并对其中13例早发性阿尔茨海默病(Eoad)、30例迟发性阿尔茨海默病(LOAD)和30例血管性痴呆(VD)患者进行了载脂蛋白E(Apo E)表型分析。用等电聚焦和免疫印迹法检测载脂蛋白E的表型。统计分析采用_X^2检验，各载脂蛋白E表型频率在性别和年龄上均无显著差异，估计在正常对照组和老年对照组中，epsilon 2、epsilon 3和epsilon 4的频率分别为0.05、0.85和0.10。载脂蛋白E表型频率在60岁及以上老年对照组与60岁及以上老年对照组之间差异有统计学意义(P&lt；0.0001)，而老年对照组与EOAD或VD组之间差异无统计学意义。EOAD组、LOAD组和VD组的epsion3频率分别为0.77、0.60和0.81；epsilon 4的频率分别为0.19、0.38和0.13。在LOAD组中，epsilon 3频率显著低于对照组(p<0.0001)，而epsilon 4频率显著高于对照组(p&lt；0.0001)。

To clarify the association of apolipoprotein E4 (apo E4) with the pathognenesis of Alzehimer's disease (AD) , we analyzed the distribution of apolipoprotein E (apo E) phenotype and the ealculated the frequency of epsilon2, epsilon4 of 692 healthy controls and 75 demented patients from the inhabitants in the middle and nothern areas of Mie Prefecture of Japan.Demented subjects included 13 patients with early onset Alzheimer's desease group (EOAD) , 30 patients with late onset Alzheimer's disease group (LOAD) , and 30 patients with vascular dementia group (VD). The apolipoprotein E phenotype was determined by the isoelectric focusing and immunoblotting. The_X^2 test was used in statical analysis.There were no significant differences between sexes or among ages in the frequency of each apo E phenotype, and the estimated frequency of epsilon2, epsilon3, and epsilon4 was 0.05,0.85 and 0.10 respectively in normal and elderly controls. There was a significant difference in the frequency of apo E phenotypes between the patients with LOAD and elderly controls aged 60 years or older (p<0.0001) , but there was no statiscal difference between the elderly controls and EOAD or VD group. The frequency of epsilon3 was 0.77 in EOAD,0.60 in LOAD and 0.81 in VD,and the frequency of epsilon4 was 0.19 in EOAD,0.38 in LOAD and 0.13 in VD.In the patients with LOAD,epsilon3 frequency was significantly lower (p<0.0001) and epsilon4 frequency was significantly higher (p<0.0001) than that in the controls.The frequency of apo E4 was significantly high in patients with LOAD,and the apo E4 is a possible candidate of one of the risk factors of development of LOAD.