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An Immunohistochemical Study On Neurofibrillary Tangles and Senile Plaques Of Demented And Non-demented Old People ; a semiquantative analysis with computed image analyzer

痴呆和非痴呆老年人神经原纤维缠结和老年斑的免疫组织化学研究；

基本信息

批准号：
03454240
负责人：
KUZUHARA Shigeki
金额：
$ 3.78万
依托单位：
MIE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

The temporal lobe sections of the autopsy brains from 161 non-demented controls aged 50s-100s and from 36 cases of senile dementia of Alzheimer's type (SDAT) were immunohistochemically stained with tau protein for Alzheimer's neu rofibrillary tangles (NFTs) and with beta-protein for amyloid senile plaques (SPs). NFTs and SPs were semiquantatively counted with eyes. The findings were as follows :1)In the controls, many NFTs appeared in the hippocampus (HC) and parahippocampal gyrus (PHG) in the aged subjects and the number increased as they became older, but there were very few in the neocortical gray matter areas. IN SDAT cases, in contrast, many or considerable number of NFTs were seen in the neocortical gray matter areas as well as abundant ones in HC and PHG.2)In all SDAT cases, there were many of abundant SPs in the cortical gray matter areas. In controls, however, there were many or abundant SPs in some cases, but few or none in others.3)In both controls and SDAT cases, NFTs preferentially affected HC and PHG ; whereas SPs, PHG and neocortical gray matter.These findings suggest that presence of many NFTs in the neocortical gray matter related aomost always with clinical dementia but presence of many SPs were not always related with dementia. The trial to semiquantatively evaluate NFTs and SPs with the computed image analyzer is now in progeress.

对161例50 ~ 100岁非痴呆对照者和36例Alzheimer型老年性痴呆（SDAT）患者的尸检脑颞叶切片进行了Tau蛋白和β蛋白的免疫组化染色，分别检测Alzheimer神经元缠结（NFT）和淀粉样老年斑（SP）。用眼半定量计数NFT和SP。结果表明：（1）对照组中，老年组海马（HC）和海马旁回（PHG）出现较多NFT，且随年龄增长而增多，而新皮质灰质区NFT较少;在SDAT病例中，NFTs在新皮质灰质区较多或相当数量，在HC和PHG中也有丰富的NFTs。2）在所有SDAT病例中，皮质灰质区都有丰富的SP。3）在正常对照组和SDAT组中，NFTs均优先累及HC和PHG，而SP、PHG和新皮质灰质，提示新皮质灰质中NFTs的增多与临床痴呆的发生密切相关，而SP的增多与痴呆的发生不一定相关。用计算机图像分析仪半定量评估NFT和SP的试验正在进行中。

项目成果

期刊论文数量（62）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Kuzuhara S: "Advances in Neurology vol.60" Raven Press, 6 (1993)

葛原 S：“神经学进展第 60 卷”Raven Press，6 (1993)

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Yoshimura M,Yamanouchi H,Kuzuhara S,Mori H,Sugiura S,Mizutani T,Shimada H,Tomonaga M,Toyokura Y: "Dementia in cerebral amyloid angiopathy:a clinicopathological study." J Neurol. 239. 441-450 (1992)

Yoshimura M，Yamanouchi H，Kuzuhara S，Mori H，Sugiura S，Mizutani T，Shimada H，Tomonaga M，Toyokura Y：“脑淀粉样血管病中的痴呆：临床病理学研究。”

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Kuzuhara S: "Neurofibrillary tangles and dementia of Alzheimer type" Dementia (Tokyo). 8. 58-64 (1994)

Kuzuhara S：“神经原纤维缠结和阿尔茨海默型痴呆”痴呆症（东京）。

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葛原茂樹: "アミロイド沈着と痴呆との関連について." 循環科学. 12(10). 958-962 (1992)

Shigeki Kuzuhara：“淀粉样蛋白沉积与痴呆之间的关系。”12（10）（1992）。

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吉村正博,名倉博史,濱本真,山之内博,伊藤雄二,宮崎徳蔵,葛原茂樹,豊倉康夫: "脳卒中と痴呆;アルツハイマー型痴呆との鑑別は?" Dementia. 6. 395-407 (1992)

Masahiro Yoshimura、Hiroshi Nagura、Makoto Hamamoto、Hiroshi Yamanouchi、Yuji Ito、Tokuzo Miyazaki、Shigeki Kuzuhara、Yasuo Toyokura：“中风和痴呆；如何区分其与阿尔茨海默氏痴呆？” 6. 395-407 (1992)。