Research on Therapeutic Mechanisms of Ultraviolet Therapy

1.银屑病光化学疗法（PUVA）联合维生素D3软膏治疗寻常型银屑病的临床研究一项左右对照研究表明，PUVA +他卡西醇软膏（TL）优于PUVA +类固醇软膏（ST）或单独赋形剂（V）。组织学和免疫组化研究表明，PUVA + TL治疗后，皮损中表皮厚度、Ki-67+细胞、浸润白细胞和细胞粘附分子表达的增加程度或数量均比PUVA + ST或V治疗后明显减少，PUVA + TL的治疗机制可能与抗增殖和免疫调节作用有关.环孢素A（CyA，5-50 mg/kg/d）或溶媒（V）单独经口给BALB/c小鼠5天，并将小鼠耳暴露于UVB（100-500 mJ/cm 2）。UVB照射后24 h，5-溴脱氧尿苷（BrdU）+细胞数增加。CyA，但不是单独的V，显着抑制UVB诱导的BrdU+细胞的增量。Cya+UVB处理组的郎格罕细胞（LC）计数较单独处理组显著降低。CyA和UVB可以合作抑制表皮增殖和LC计数增加，这两者都是银屑病的部分原因。

1. Clinical Research on Therapeutic Mechanisms of Psoraren Photochemotherapy (PUVA) Combined With Vitamin D3 Ointment in Psoriasis VulgarisA right-left comparative study in psoriasis vulgaris demonstrated that PUVA + tacalcitol ointment (TL) was better than PUVA + steroid ointment (ST) or vehicle (V) alone. Histological and immunohistochemical studies showed that the increased number or degree of epidermal thickness, Ki-67+ cells, infiltrating leukocytes, and cell adhesion molecule expression in pretreated lesion was reduced to a greater extent after PUVA + TL than after PUVA + ST or V.Therapeutic mechanisms of PUVA + TL may be attributed to both antiproliferative and immunomodulatory effects.2. Basic Research on Therapeutic Mechanisms of Cyclosporin A Combined With Ultraviolet-B-PhotocherapyCyclosporin A (CyA,5-50 mg/kg/day) or vehicle (V) alone was administered per os to BALB/c mice for 5 days, and mouse ears were exposed to UVB (100-500 mJ/sq cm). UVB radiation inceased the number of 5-bromodeoxyuridine (BrdU)+ cells at 24h after transient suppression. CyA,but not V alone, significantly suppressed UVB-induced increment in BrdU+ cells. Cya+UVB significantly decreased Langerhans cell (LC) coants than each treatment alone. CyA and UVB may cooperate to suppress increased epidermal proliferation and LC counts, both of which are partly responsible for psoriasis.