LUTEAL REGRESSION AND PROTEINASE,APOPTOSIS,HEAT SHOCK PROTEIN

我们用大鼠黄体和人黄素化颗粒细胞研究了黄体退化的机制。我们在这2年中得到了三个不同的发现：第一，催乳素（PRL）降低了大鼠结构性黄体溶解中金属蛋白酶组织抑制因子（TIMP）mRNA的表达;如我们以前报道的，基质金属蛋白酶（MMP）-2在PRL诱导的结构性黄体溶解中显著升高。MMP-2的活性在体内受TIMP表达的调节。TIMP-1 mRNA表达减少被认为在体内提高MMP-2活性。这些结果表明，细胞外基质的降解与大鼠结构性黄体溶解的机制有关。在PRL诱导的结构性黄体溶解中，细胞发生凋亡。功能性黄体溶解不发生细胞凋亡。结果表明，大鼠黄体结构退化过程中的失重与细胞凋亡（程序性细胞死亡）有关。 ...更多信息 IDE降低人黄素化颗粒细胞的ATP水平。我们报道过，H_2O_2处理降低人黄体化颗粒细胞cAMP和孕酮的产生。我们研究了ATP的耗竭可能与cAMP合成的抑制有关。而3-氨基苯甲酰胺，这是一种聚（ADP-核糖）聚合酶的抑制剂，阻止过氧化物诱导的ATP耗竭，过氧化物减少hCG刺激的cAMP的生产。前列腺素F_2_<alpha>（PGF）激活大鼠黄体细胞的磷脂酶D（PL D），我们以前报道过PGF激活大鼠黄体细胞的PLA_2和PLC。PCL激活与前列腺素F诱导的黄体溶解无关。我们用油酸标记的大鼠黄体细胞在乙醇存在下研究PLD激活是否可能是PGF诱导的黄体溶解的第二信号，结果表明PLD激活可能是PGF处理的大鼠黄体细胞的第二信使。少

We investigated the mechanism of luteal regression using rat corpus luteum and human luteinized granulosa cells. We got three different findings these 2 years.First, Tissue inhibitor of metalloproteinase (TIMP) mRNA expression was reduced by prolactin (PRL) in structural luteolysis of the rat. As we reported before, matrix metallopreoteinase (MMP)-2 was significantly increased in PRL-incuced structural luteolysis. Actural MMP-2 activity is well known to be regulated by TIMP expression in vivo. Reduced TIMP-1 mRNA expression is thought to elevate MMP-2 activity in vivo. These results revealed that degradation of extracellular matrix is associated with the mechanism of structural luteolysis in the rat. Apoptosis was occured in PRL-induced structural luteolysis in the rat. We also showed apoptosis did not occured in functional luteolysis. Thease results showed that the loss of weight in structural involution of rat corpus luteum is related to apoptosis (programed cell death).Second, perox … More ide reduced ATP levels of human luteinized granulosa cells. As we reported, H_2O_2 treatment reduced cAMP and progesterone production in human luteinized granulosa cells. We investigated that depletion of ATP may be related to the inhibition of cAMP synthesis. While 3-aminobenzamide, which is an inhibitor of poly (ADP-ribose) polymerase, blocked peroxide-induced ATP depletion, peroxide reduced hCG-stimulated cAMP production. Thease results indicated that ATP depletion is not associated with peroxide-reduced cAMP synthesis.Third, prostaglandin F_2_<alpha> (PGF) activated phospholipase (PL) D in rat luteal cells.We raported before that PGF activated PLA_2 and PLC in rat luteal cells. PCL activation is not reported to be associated with PGF-induced luteolysis. We examined if PLD activation may be the second signal of PGF-induced luteolysis using oleic acid-labeled rat luteal cells under the presence of ethanol.The result showed that PLD activation may be second messenger in PGF-treated rat luteal cells. Less