Establishment of the rapid immunohistochemical staining for proliferating cell nuclear antigen in oral cancer and jaw bone.

研究了一种快速一步免疫组织化学染色方法--增强型聚合物一步法在福尔马林固定石蜡包埋的舌鳞癌切片中检测增殖细胞核抗原(EPOS-PCNA)。采用免疫组织化学ABC法，比较有无高压灭菌处理后Epos-PCNA与免疫组织化学染色结果。EPOS-PCNA标记指数(标记细胞占细胞总数的百分比)与高压灭菌处理的免疫组织化学方法有显著相关性。我们的结论是，这种方法是一种可靠的、省时的技术，在定量组织病理学和进行双重免疫染色时将是有用的。在大鼠下颌骨中，由于肥大的软骨细胞层仍有非特异性染色，应尝试合适的封闭剂。

A rapid one-step immunostaining method, enhanced polymer one-step staining for proliferating cell nuclear antigen (EPOS-PCNA) in formalin-fixed, paraffin-embedded sections of tongue squamous cell carcinoma was investigated. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complax (ABC) method with or without autoclave treatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol with autoclave pretreatment. We conclude that this method is a reliable, time-sparing techniqne that would be useful in quantitative histopathology and in performing double immunostaining. In the rat mandibular condyle an appropriate blocking reagent should be tried, because we still observed non-specific staining in the layr of hypertrophic chondrocyte.