Basic and Clinical Studies about the Spectroscopical Diagnostic Technology of Ortho-Oral Diseases

在光谱诊断技术中，荧光光谱诊断和在可见波长区域中用活组织检查取样的染色组织的诊断被很好地用作病理检测。本课题研究的光谱诊断技术，旨在将冰冻切片中我们无法探测到的成分在红外波段成像作为一种新的诊断技术。[1]龋病的拉曼显微光谱测量：（1）龋牙釉质和牙本质的边缘区位于羟基磷灰石中PO_4 ~+的P=O对称伸缩峰<2->上。结果表明，牙釉质区羟基磷灰石钙含量比牙本质区高2倍。(ii)龋损区牙釉质和牙本质中羟基磷灰石钙的含量均下降。(iii)观察到同一边界地区 关于我们 通过扫描电子显微镜（SEM）和X射线（EDX）探测器的P和Ca原子元素分布来获得牙釉质和牙本质之间的图像对比度。拉曼图谱显示了釉质区羟基磷灰石钙分布的图像对比度，而EDX图谱中P、Ca原子的分布对比度不明显。[2]唾液杯的拉曼显微光谱测量：（i）在通过拉曼显微光谱仪测量唾液杯的结果中，在钙化区域观察到磷酸（P=O）吸收峰，并且峰的映射显示为环，并且环的中心区域脱离钙化。(ii)用傅立叶变换红外光谱仪的衰减全反射（ATR）法测定唾液杯患者唾液干燥过程中的含量。利用傅立叶变换红外光谱分析软件对唾液蛋白的第二构象组分进行分析。在正常唾液条件下，随着干燥时间的延长，各组分间的良片率逐渐增大或减小。少

Fluorescence spectroscopic diagnosis and a diagnosis of staining tissue sampled with a biopsy in the region of visible wavelength were used well as a pathological detection in the spectroscopic diagnosis technology. In this projected studies of spectroscopic diagnosis technique, it is the aim to take imaging of the components in the frozen section in the field of infrared region which we can not detect as a new diagnosis technique. In the results, phenomena and knowledge were observed during two years as follows :[1]Raman Microspectroscopic Measurements of Caries : (i) The border area of enamel and dentin on a caries tooth were mapping at the peak of P=O symmetrical stretching component of PO_4^<2-> of calcium hydroxyapatites. It resulted that the content of calcium hydroxyapatites in enamel area was higher two times than that in dentin. (ii) The content of calcium hydroxyapatites was dropped out in caries region on both enamel and dentin areas. (iii) The same border area was observed … More by a scanning electron microscopy (SEM) and an element distribution of X-ray (EDX) detector of P and Ca atoms to get an image contrast between enamel and dentin. Raman mapping image got an image contrast in the calcium hydroxyapatites distribution in enamel area instead of the EDX images of P and Ca atoms which the distribution contrast were not clear.[2]Raman Microspectroscopic Measurements of Salivary Caliculus : (i) In the results of measurement of salivary caliculus by a Raman microspectroscope, it was observed the phosphoric acid (P=O) absorption peak at the calcified area, and the mapping of the peak showed a ring and the center area of ring was dropped out of the calcification. (ii) The saliva liquid of the patient for salivary caliculus was measured by an ATR method of FT-IR spectrophotometer during drying the sample. The second conformation components of the saliva liquid protein were analyzed the FT-IR spectrum by a software. The Better sheet component ratio against the other ones increased with drying, which were different of decrease in the case of normal saliva liquid. Less