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Basic and Clinical Studies about the Spectroscopical Diagnostic Technology of Ortho-Oral Diseases

口腔正畸疾病光谱诊断技术的基础与临床研究

基本信息

批准号：
15592102
负责人：
SANO Kazuo
金额：
$ 2.05万
依托单位：
University of Fukui (2004)福井医科大学 (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592102/
关键词：
Raman Microspectroscopic measurements Caries Analysis of Saliva Analysis of Salivary Caliculus Calcium Salt of Phophoric Acid Symmetric Stretching of P=O Feurier-Transform Infrared Microspectroscopic Method Secondary Component Ratio of Prote

项目摘要

Fluorescence spectroscopic diagnosis and a diagnosis of staining tissue sampled with a biopsy in the region of visible wavelength were used well as a pathological detection in the spectroscopic diagnosis technology. In this projected studies of spectroscopic diagnosis technique, it is the aim to take imaging of the components in the frozen section in the field of infrared region which we can not detect as a new diagnosis technique. In the results, phenomena and knowledge were observed during two years as follows :[1]Raman Microspectroscopic Measurements of Caries : (i) The border area of enamel and dentin on a caries tooth were mapping at the peak of P=O symmetrical stretching component of PO_4^<2-> of calcium hydroxyapatites. It resulted that the content of calcium hydroxyapatites in enamel area was higher two times than that in dentin. (ii) The content of calcium hydroxyapatites was dropped out in caries region on both enamel and dentin areas. (iii) The same border area was observed … More by a scanning electron microscopy (SEM) and an element distribution of X-ray (EDX) detector of P and Ca atoms to get an image contrast between enamel and dentin. Raman mapping image got an image contrast in the calcium hydroxyapatites distribution in enamel area instead of the EDX images of P and Ca atoms which the distribution contrast were not clear.[2]Raman Microspectroscopic Measurements of Salivary Caliculus : (i) In the results of measurement of salivary caliculus by a Raman microspectroscope, it was observed the phosphoric acid (P=O) absorption peak at the calcified area, and the mapping of the peak showed a ring and the center area of ring was dropped out of the calcification. (ii) The saliva liquid of the patient for salivary caliculus was measured by an ATR method of FT-IR spectrophotometer during drying the sample. The second conformation components of the saliva liquid protein were analyzed the FT-IR spectrum by a software. The Better sheet component ratio against the other ones increased with drying, which were different of decrease in the case of normal saliva liquid. Less

在光谱诊断技术中，荧光光谱诊断和在可见波长区域中用活组织检查取样的染色组织的诊断被很好地用作病理检测。本次光谱诊断技术研究的目的是将冰冻切片中我们无法检测到的成分在红外区域成像作为一种新的诊断技术。[1]龋病的拉曼显微光谱测量：（1）龋牙釉质和牙本质的边缘区位于羟基磷灰石中PO_4 ~+的P=O对称伸缩峰<2->上。结果表明，牙釉质区羟基磷灰石钙含量比牙本质区高2倍。(ii)龋损区牙釉质和牙本质中羟基磷灰石钙的含量均下降。(iii)观察到同一边界地区 ...更多信息通过扫描电子显微镜（SEM）和X射线能谱仪（EDX）测定牙釉质和牙本质中P、Ca原子的含量，得到牙釉质和牙本质的图像对比。拉曼图谱图像得到了牙釉质区钙羟基磷灰石分布的图像对比度，而不是P和Ca原子的EDX图像，其分布对比度不清楚。[2]唾液杯的拉曼显微光谱测量：（i）在通过拉曼显微光谱仪测量唾液杯的结果中，在钙化区域观察到磷酸（P=O）吸收峰，并且峰的映射显示为环，并且环的中心区域脱离钙化。(ii)用傅立叶变换红外光谱仪的衰减全反射（ATR）法测定唾液杯患者唾液干燥过程中的含量。利用傅立叶变换红外光谱分析软件对唾液蛋白的第二构象组分进行分析。在正常唾液条件下，随着干燥时间的延长，各组分间的良片率逐渐增大或减小。少