Study of the low molecular weight GTP binding proteins in amylase exocytosis of parotid acini in vitro.

三聚体GTP结合蛋白在质膜上起信息和/或转化因子的作用。但存在低分子量GTP结合蛋白，表达于细胞质组分中。此外，它是插入在分泌细胞的分泌颗粒膜和作为组成的成员。腮腺腺泡细胞低分子量GTP结合蛋白存在于分泌颗粒膜中，与淀粉酶分泌有关。我们检查了是否玩它。采用Hopfer等人描述的percol -gradient离心机分离大鼠腮腺分泌颗粒。我们在此报道，通过[32]-GTP覆盖测定，至少有21个低分子gtp结合蛋白（21 kD-25 kD）与大鼠腮腺腺泡的质膜和分泌颗粒膜相关。大鼠腮腺组织分离分泌颗粒。rho蛋白的Western blot分析。rho蛋白A和B定位于质膜和分泌颗粒膜，分子量为21 kD.2。adp -核糖素转移酶C3灭活腮腺腺腺腺的rho p21后，淀粉酶的释放受到抑制，表明rho p21介导了淀粉酶的分泌。

The trimeric GTP binding protein plays it as information and/or transformation factor on the plasma membranes. But existence of low molecular weight GTP binding protein which expressed in cytosol fraction. Futhermore, it is inserted in the secretory granule membranes in secretory cell and as member of constitution. So, low molecular weight GTP binding protein in parotid acinar cell exists in secretory granule membranes, and is which associated with amylase secretion. We examined whether playd it. Secretory granules of rat parotid glands were isolated by percoll-gradient centrifuges as described by Hopfer et al. We report here that at least 21ow molecular GTP-binding proteins (21 kD-25 kD) are associated with the plasma membrane and secretory granule membranes from rat parotid acini by [32 [-GTP overlay assay.Results1. The secretory granules isolated by rat parotid tissues. Western blotting analysis of the rho proteins. The rho protein A and B localized the plasma menbranes and secretory granule membranes as molecular weight at 21 kD.2. Inactivation of rho p21 of parotid acini by ADP-rybosiltransferase C3 resulted in the inhibited release of amylase, suggesting that amylase secretion was mediated by rho p21.