Study for the role of microcirculation for exocrine and endocrine functions of pancreas.

应用双点显微光谱技术研究了微循环对小鼠胰腺外分泌和内分泌功能的影响。这项研究于1994年至1995年进行。<Results in 1994>（1）单微血管O_2输运参数分析装置的构建和方法的建立。10 μ m直径）成功地与麻醉大鼠肝脏和灌流离体肝脏。(2)应用上述技术研究了微循环对胰腺功能的作用：1）首次获得了胰腺外分泌微血管的O_2转运参数和O_2释放速率，2）将O_2释放速率与胰腺外分泌功能耦合起来。<Results in 1995>（1）O_2释放速率与饮食密切相关，而红细胞速度、血红蛋白氧合和血管内血红蛋白浓度与饮食无关。(2)抑制糖酵解可显著降低小鼠胰腺O_2释放速率，其机制有待进一步研究。

The role of microcirculation for the exocrine and endocrine functions of murine pancreas was investigated with dual-spot microspectroscopy. The study was performed from 1994 to 1995.<Results in 1994>(1) Construction of apparatus and establishment for analytical method of O_2 transport parameters in single microvessels (ca. 10 um in diameter) were succeeded with anesthetized rat liver and perfused isolated liver.(2) The above technique was applied to the investigation for the role of microcirculation for the pancreatic functions : 1) O_2 transport parameters and rate of O_2 release from exocrine pancreatic microvessels were obtained for the first time, and 2) the rate of O_2 release were coupled with the exocrine function.<Results in 1995>(1) The rate of O_2 release strongly depended on a diet, but red blood cells velocity, oxygenation of hemoglobin and intravascular hemoglobin concentration did not.(2) The rate of O_2 release was significantly reduced by inhibition of glycolysis in the murine pancreas.The mechanisms for these results should be further investigated in detail.