Molecular mechanism for adsorption and adsorption of soil components formed on the surface of equipment wall during manufacturing and development of an efficient cleaning method

在各种制造过程中，用于制造各种产品的设备和管道系统的清洗是保持设备卫生条件和/或功能的必要条件。特别是，在食品和生物产品的制造过程中，除了时间之外，通常还要消耗大量的能源和洗涤剂，因为产品的土壤或残留物与设备表面有强烈的相互作用。在本研究中，我们研究了各种蛋白质/多肽和低分子量物质作为模型土壤组分在各种金属氧化物、塑料和玻璃表面的吸附机理。通过吸附/解吸实验、红外光谱分析和分子计算，明确了金属氧化物表面pI值/溶液ph值对吸附特性的影响，以及吸附蛋白质的构象变化，阐明了可逆/不可逆吸附的分子机理、吸附分子的状态和取向、吸附蛋白质表面pI值对吸附特性的影响。采用本课题组开发的h2o2 -电解清洗法，考察了共存盐的种类及其浓度等因素对金属氧化物表面蛋白质污染去除率的影响。此外，我们在研究蛋白质吸附机理的过程中，开发了一种定向和结构可控的蛋白质固定化方法，用于芯片生物技术的各种应用。

In various manufacturing processes, cleaning of the equipment and piping systems used for manufacturing of various products is requisite for maintaining sanitary conditions and/or functions of the equipments. In particular, in the food- and bio-products manufacturing processes, a large amount of energy and detergents are usually consumed in addition to time, since soils or residues of the products strongly interact with the equipment surface. In this study, we studied adsorption mechanisms of various proteins/peptides and low-molecular weight substances as a model soil component on the surfaces of various metal oxides, plastics, and glasses. We clarified the molecular mechanisms for reversible/irreversible adsorptions, states and orientation of the molecules adsorbed, the effects of the pI values of the metal oxide surfaces/solution pHs on the adsorption characteristics, and conformational change of the adsorbed proteins, on the basis of adsorption/desorption experiments, IR spectrum analyses, and molecular calculations. Furthermore, we investigated the effects of various factors such as the kinds of the co-existing salts and their concentrations on the removal rates of the metal oxide surface fouled with proteins, using H2O2-electrolysis cleaning method that was developed by the author's group. Furthermore, we developed a method to immobilize proteins with controlled orientation and structure for various uses in chip-based biotechnology, which was derived in the course of investigation on the adsorption mechanism of proteins.