Eco-genome study of adaptive genes in Primula sieboldii

为了制定濒危野生报春花的保护策略，我们试图通过QTL分析和基于表达遗传信息的全基因组基因分析来寻找适应基因。对影响群体延续的几个与异源花柱相关的基因区域进行了高精度检测。通过比较野生报春花和栽培报春花的遗传多样性，明确了驯化过程中的瓶颈效应。利用EST分子标记对北海道至关东地区7个群体的现蕾期、开花期等适应性性状进行了遗传分析。在迎春花的适应过程中，我们可以检测到许多与发芽有关的位点。我们的研究结果不仅将有助于保护策略的发展，而且将成为展示如何利用基因组信息进行保护策略的模型。

To develop the conservation strategy of endangered wild primroses, we tried to identify the adaptive genes by QTL analysis and the genome-wide gene analysis based on the expression genetic information. Several associated gene regions about hetero style which affected the continuation of the group were detected with high precision. We clarified that a bottle neck effect committed the process of domestication by a comparison between genetic diversity of wild primrose and cultivars. Using EST markers, genetic analysis about the adaptive characters including the budding date and the flowering time were performed for 7 populations from Hokkaido to Kanto region. We could detect a lot of loci about budding in conjunction with the adaptation of the primrose. The results of our study will contribute not only the development of the conservation strategy, but also will become the model which show how to use genome information for conservation strategy.