The construction for recycle of fluorine resource with the complex method

我们已经分离到能够降解含氟物质的细菌，有8株放线杆菌表现出对二氟乙酸乙酯(DFAC)的降解能力，细菌积累了二氟乙酸乙酯，然后给出了氟离子。此外，13株放线菌表现出对氟苯和/或三氟化苯的降解性。在分批培养中，菌株在含氟材料上的生长导致氟离子的释放。以三磷酸腺苷和L蛋氨酸为原料，在氟离子存在下，用S-腺苷-L-蛋氨酸合成酶、氟化酶和甲硫基腺苷核苷酶，建立了一锅三步连续酶促合成5-氟-5-脱氧-D-核糖。特别是，为了氟化学的可持续发展，我们建立了从BF4离子液体中产生的氟离子的再利用和/或一锅法合成5-FDR过程中BTF的生物降解。此外，我们还建立了生物降解BTF产生的氟离子再转化为氟化钙的方法。

We have found the isolation of bacterial strains capable of degrading fluorinated materials, 8 strains of Actinobacteria exhibited degradability of ethyl difluoroacetate(DFAc) was accumulated by bacteria, giving difluoroacetic acid and then fluoride ion. Further, 13 strains of Actinobacteria exhibited degradability of fluorobenzene and/or benzotrifluoride. In batch culture, growth of strains on fluorinated materials led to the release of fluoride ion. We have established the one-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-D-ribose(5-FDR) from ATP and L-methionine using S-adenosyl-L-methionine synthase(MetK), fluorinase and methylthioadenosinenucleosidase(MtnN) in the presence of fluoride ion. Especially, for the purpose of thesustainable development of fluorine chemistry, we have established the reuse of fluoride ion generated from BF4 ionic liquids and/or the biodegradation of BTF in the one-pot synthetic process to 5-FDR. Further, we have established the retransformation of fluoride ion generated from biodegradation of BTF to calcium fluoride.