Visuallization of the spatio-temporal activities of Rab GTPases forming Rab cascades

在酿酒酵母中，Rab GTP酶级联作用于ER到高尔基体分泌途径的调节。Ypt1p参与内质网到顺式高尔基体的转运，作用于Ypt31/32p的上游，介导来自反高尔基体的后高尔基体小泡的萌发。Ypt31/32P位于Sec4p的上游，Sec4p是一种与高尔基体后分泌囊泡相关的Rab GTP酶。在这项研究中，我开发了这些Rab GTP酶的分子内FRET探针，以可视化活的酵母细胞中的时空活动。

Rab GTPase cascade in the regulation of ER-to-Golgi step of the secretory pathway was proposed in Saccaromyces cerevisiae. Ypt1p involved to ER to cis-Golgi transport acts upstream of the Ypt31/32p, mediating the budding of post-Golgi vesicles from the trans-Golgi. Ypt31/32p acts upstream of Sec4p, the Rab GTPase associated with post Golgi derived secretory vesicles. In this study, I developed the intra molecular FRET probes of these Rab GTPases to visualize the spacio-temporal activities in the living yeast cells.