Development of novel DNA sensor by nanoimprint technique

近年来，DNA的性质被认为是理解新的功能生物器件的基础。然而，大多数关于DNA纳米图案化的报道并不是为了制造它们。因此，我使用纳米压印工艺进行了DNA纳米图案化。将涂覆有聚-L-溶素的基底在120 °C下加热，并且纳米压印进行120 °C、6 MPa、5分钟，并且其在基底中施加用聚-L-溶素固定的lmg/ml DNA溶液。最后，用两次水和一次热水强烈地洗涤基底。获得了由线宽为约700 nm的线组成的DNA线，并且非常精细的线对应于模具表面的凸起图案。这些结果表明，压印将使聚-L-赖氨酸改革暴露在高压和高温下。因此，由于DNA是用多聚赖氨酸中的氨基固定的，大量的氨基会通过印迹暴露在表面，形成图案化的DNA。

The DNA properties were considered recently to understand new functional bio-device. However, most reports about the DNA nano patterning are not intended to make them. Therefore, I carried out DNA nano patterning using a nanoimprint process. The substrate coated with poly-L-lysin was heated at 120 °C and the nanoimprint carried it forth for 120 °C, 6 MPa, 5 min, and it applied 1 mg/ml DNA solution fixed with poly-L-lysin in substrates. Finally the substrate was washed at twice of the water and once of the hot water intensely. DNA lines that consist of lines at about 700 nm as line width was obtained, and the very fine lines correspond to convex patterns of the mold surface. These results suggest that the imprint would make the poly-L-lysine reform by exposing it under high pressure and high temperature. Therefore since DNA is immobilized with the amino group in the poly-L-lysine, a lot of amino group would expose on the surface by the imprint from the balk, and would be patterned DNA.