Development of the reverse genetics system for Rice stripe virus

水稻条纹病毒（RSV）是一种通过棕色小飞虱传播的侵染水稻植株的负链RNA病毒。由于缺乏反向遗传系统，关于植物负链RNA病毒如何繁殖的知识是有限的。在这个项目中，我试图开发一个RSV的反向遗传系统。为了重建感染性核糖核蛋白（RNP）复合体，我成功地表达和纯化了RSV的核衣壳蛋白和RNA聚合酶，它们是RSV RNP的组成部分。虽然这些蛋白与病毒RNA的混合没有形成感染性RNP，但本项目获得的材料将有助于进一步发展植物负链RNA病毒的研究。

Rice stripe virus (RSV) is a negative-strand RNA virus that infects Rice plants transmitted by small brown plant hopper. Due to the unavailability of reverse genetics systems, knowledge about how plant negative-strand RNA viruses multiply is limited. In this project, I tried to develop a reverse genetics system of RSV. To reconstitute an infectious ribonucleoprotein (RNP) complex, I succeeded to express and purify the nucleocapsid protein and RNA polymerase of RSV, components of RSV RNP. Although mixtures of these proteins with viral RNA did not result in formation of the infectious RNP, the materials obtained in this project would help further development of the study of plant negative-strand RNA viruses.