The development of therapy for inflammatory bal tissue with Paneth cell-protection factorsowel disease using cultured epitheli

本研究旨在分析肠道隐窝结构再生的机制，建立维持正常隐窝结构的新型培养体系。本研究从小鼠小肠中剥离肠绒毛和隐窝，收集富含隐窝的部分进行培养。小肠隐窝在37度的马氏凝胶中混合，并覆盖干细胞选择培养基。小肠隐窝不进行细胞分离培养。考察了培养基中的几个因素，发现R-spondin是影响培养系统的关键因素。为了建立动物模型，对狗和猪的组织进行了病理分析。富含颗粒的Paneth细胞在猪肠中可见，但在狗肠中可见。猪是替代人类研究的候选动物模型之一。未来动物模型的发展备受期待。

The aim of this research is to analyze the mechanisms in regeneration of crypt structures in the gut, and to establish novel culture system with maintaining the normal crypt structure.In this study, intestinal villi and crypt are exfoliated from mouse small intestine and the crypt-rich fractions are collected for the culture. Small intestinal crypts are mixed in Marti-gel at 37 degree, and covered with stem cell selection media. The small intestinal crypts are cultured without cell isolation. Several factors in culture media are checked and R-spondin is found to be a critical factor for culture system.To establish the animal model, the tissues of dog and pig are analyzed pathologically. Paneth cells with rich granules are observed in the porcine intestine but in the dog. Pig is one of the candidates for animal model alternative to human research. The development of animal model is highly expected in future.