Identification of genetic factors affect the success or failure of the transformation in barley

我们探索了基因组区域所需的遗传转化，通过使用一个映射群体产生的大麦品种。金诺，这是一个可靠的品种转化。其结果是，估计染色体No.1H、No.2H、No.3H、No.6H和No.7H的部分区域为候选基因的位置。然后，我们分离了4308个基因的表达模式是特定的黄金承诺，通过全面的基因表达谱芯片分析。激素分析表明，生长素对大麦愈伤组织的再生有促进作用，而脱落酸和水杨酸对大麦愈伤组织的再生有抑制作用。

We explored the genomic regions required for genetic transformation by using a mapping populations generated from barley cv. Golden Promise, which was a reliable cultivar for transformation. As a result of this, the partial region of chromosome No. 1H, No. 2 H, No. 3H, No. 6 H and No. 7 H were estimated as the positions of candidate genes. Then we isolated 4308 genes which expression patterns were specific in Golden Promise by a comprehensive gene expression analysis using microarray. In the analysis of phytohormone, we identified that auxin showed a positive effect in regeneration of barley callus, although abscisic acid and salicylic acid showed negative.