The establishment of in vivo immunological monitoring by using humanized mouse model in living related liver transplant recipients.

在目前的研究中，确定了产生人源化小鼠模型并预防移植物抗宿主病的人淋巴细胞的确切数量。重要的是，人淋巴细胞是从新鲜血液中分离出来的，而不是储存在冷冻库中。并建立了适用的荧光染色淋巴细胞增殖测定方法。同时，为了检验体外监测方法的有效性，在活体相关肝移植患者中应用了可在短时间内获得结果的免疫检测方法。单一的免疫监测试验在可靠性方面存在局限性，提示人源化体内监测试验是诊断准确免疫状态的有吸引力的工具。

In the current study, the exact numbers of human lymphocyte to generate the humanized mouse model but prevent the graft versus host disease were identified. It is important that the human lymphocytes were isolated from fresh blood not stored them in deep freezer. Also the applicable lymphocytes proliferation assays by using fluorescent dye were established. Meanwhile, to examine the efficacy of in vitro monitoring assays, immune-assays possible to obtain results in a short period of time were applied in living related liver transplant patients. A single immunological monitoring test has limitations in terms of reliability suggesting that humanized in vivo monitoring assay is attractive tool to diagnose the exact immune status.