Influence of habitual exercise and circumstantial stresses on non-specific immunity and oxidant-antioxidant balance

我们在许多科学会议上介绍了使用大鼠作为实验动物的基础研究结果。应激，强迫或自愿运动，睡眠障碍，和隔离，加载到成熟的大鼠。强迫和随意运动对体重有影响，但对肺泡巨噬细胞产生活性氧的能力几乎没有影响。通过使用96孔微孔板的平行发光测量系统测量ROS。为了开发测量肺泡巨噬细胞活性的指标，对系统进行了改造以用于荧光测量。应激组大鼠肺线粒体和微粒体组分的氧化-抗氧化平衡差异不大。这些结果表明，肺的非特异性免疫和氧化-抗氧化平衡对成熟后负荷的应激具有抵抗力。 ...更多信息 运动员的心理和食物摄入状况，并将结果报告给科学期刊。在为期一个月的夏令营期间进行耐力训练对非特异性免疫的影响进行了调查，并发表在《国际运动医学杂志》上。结果表明，非特异性免疫并没有直接受到夏令营的损害。在为期一个月的长跑比赛中，慢跑者自然杀伤细胞的细胞毒活性不受耐力跑的影响。建立了一种血清总抗氧化活性的测定方法，用于评价体内氧化-抗氧化平衡，并报道了血清总抗氧化活性的机理探讨及应用。血清总抗氧化活性是一种捕获活性氧的能力，通过测定血清总抗氧化活性来评价每日运动对老年人（65岁以上）氧化-抗氧化平衡的影响，但未观察到每日运动的显著影响。日常运动的强度可能太低，不会影响氧化-抗氧化平衡。少

We presented the results of basic research using rats as an experimental animal at many scientific congresses. The stresses, forced or voluntary exercise, sleep disturbance, and isolation, were loaded to mature rats. The forced and voluntary exercise influenced body weights, however, the producing ability of reactive oxygen species (ROS) from alveolar macrophages was almost not affected by the stresses. ROS was measured by a parallel luminescent measuring system using a 96-well microplate. To develop an index to measure alveolar macrophage activity, the system was remodeled for fluorescence measurement. There were few significant differences among the stress groups on the oxidant-antioxidant balance in mitochondrial and microsomal fractions of rat lungs. These results suggest that non-specific immunity and oxidant-antioxidant balance in lungs are resistant to the stresses loaded after maturation.To clarify the subjects' circumstances toward forced or voluntary, we surveyed the health, … More psychological, and food-intake statuses in athletes and reported the results to the scientific journals. The effect of endurance training during a 1-month summer camp on the non-specific immunity was investigated and published in Int. J.Sports Med. The results suggest that the non-specific immunity is not directly impaired by the summer camp. The cytotoxic activities of natural killer cells of joggers were not affected by endurance running during a month-long distance run competition. A measurement method for a total antioxidant activity of serum was established to estimate the in vivo oxidant-antioxidant balance and we reported the mechanistic approach and application of the serum total antioxidant activity. The serum total antioxidant activity is a trapping capacity of ROS.The influence of daily-exercise on the oxidant-antioxidant balance of aged residents (over 65 years old) was estimated by serum, however, no significant influence of daily-exeercise was observed. Intensity of daily-exercise may be too low to affect the oxidant-antioxidant balance. Less