The study of meintenance of bone metabolism in small animals

利用动物模型阐明骨质疏松的病理机制，建立骨质疏松的治疗和预防方法是十分必要的。因此，澄清骨代谢，包括改变骨矿物质含量（BMC）和骨矿物质密度（BMD），是很重要的。因此，本研究采用Aloka的DCS-600R DXA测定不同饮食条件对骨量变化的影响。1998年，研究了钠（Na）摄入量对钙（Ca）和骨代谢的影响。在这个实验中，它能够通过DCS-600R分析骨密度，即使动物的骨量明显较低。此外，研究结果还表明，过量的钠摄入对钙和骨代谢有负面影响。1999年，通过测定体内（用活鼠）和体外（用解剖后提取的骨）BMC和BMD的变化，研究了牛奶对骨生长的影响。DCS-600R可准确、准确地分析幼年大鼠佩戴后的骨密度。此外，结果表明，乳汁可以显著有效和有价值的骨在生长期。2000年，用DCS-600R测定了PTH对低钙饲粮大鼠骨代谢的影响。此外，还分析了不同密度仪测定BMC和BMD的相关性。结果表明甲状旁腺素对维持骨代谢有积极作用。DCS-600R与其他密度计的测定结果呈正相关。三个实验结果表明，DCS-600R测量骨密度检测骨代谢是可靠的。

It is nessesary using animal models to elucidate of pathology of osteoporosis and to establish the ways of treatment and prevention of osteoporosis. Thus, a clarification of bone metabolism, include changing bone mineral content (BMC) and bone mineral density (BMD), is important. Therefore, in this study the effects of the different diet conditions on changing bone mass were determined using the DXA by Aloka's DCS-600R.In 1998, the influence of sodium (Na) intakes on calcium (Ca) and bone metabolism were examined. In this experiment, it was able to analyze the BMD by DCS-600R, even though the animal had a markedly low bone mass. In addition to this, the results indicated that an excess Na intakes affected negatively on Ca and bone metabolism.In 1999, the effect of milk on bone growth was examined by measurement of changing BMC and BMD in vivo (using vital rats) and in vitro (using extracted bone after dissection). The BMD of the young rats just after wearning could analyze accurately and precisely by DCS-600R.Moreover, the results suggested that the milk could be markedly effective and valuable for bone in growing period.In 2000, the influence of PTH on bone metabolism in the rats fed low Ca diet was determined using the DCS-600R.In addition, the correlation of the results of BMC and BMD in different densitometer was also examined. The results indicated that the PTH could effect positively on meintaining bone metabolism. And there was a possitive correlation between the results of DCS-600R and other densitometer.The results of three experiments show the measurement of BMD by DCS-600R is reliable to examine bone metabolism.